Detection of HTLV-III/LAV antigens in peripheral blood lymphocytes from patients with AIDS |
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| Authors: | D. D. Pekovic J. -P. Chausseau N. Lapointe J. Michaud S. Garzon H. Strykowski C. Tsoukas N. Gilmore H. Goldman M. Gornitsky M. Popovic J. -M. Dupuy |
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| Affiliation: | (1) Institut Armand-Frappier, Université du Québec, Montreal, Canada;(2) Hôpital Ste-Justine, Université de Montréal, Montreal, Canada;(3) Faculté de Médecine, Université de Montréal, Montreal, Canada;(4) Montreal General, Royal Victoria, Montreal Children's, and Jewish General Hospitals, McGill University, Montreal, Canada;(5) N.I.H., Bethesda, Md., U.S.A. |
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| Abstract: | Summary HTLV-III was searched for in frozen sections of peripheral blood lymphocytes obtained from AIDS patients by an immunofluorescence technique. Human IgG against HTLV-III/LAV and monoclonal antibodies against HTLV-III/LAV P24 antigen, yielded a strong cytoplasmic fluorescence in frozen sections of the lymphocytes. Some cells containing HTLV-III antigens displayed multinucleated giant forms. They also reacted with monoclonal antibodies against helper/inducer T-cells (OKT 4+), as demonstrated by direct double staining immunofluorescence. Similarly, complexes of immunoglobulins and C3 component of complement were also detected on HTLV-III/LAV Ag expressing lymphocytes.Immunofluorescence study of frozen sections of peripheral blood lymphocytes appeared to be a simple, fast and reliable method for detection of HTLV-III/LAV Ag in AIDS patients.With 3 Figures |
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