| 尼莫地平对过氧化氢诱导人血管内皮细胞凋亡的影响 |
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| 引用本文： | 刘立新,王迪浔,哈斯朝鲁,杜永成. 尼莫地平对过氧化氢诱导人血管内皮细胞凋亡的影响[J]. 中国老年学杂志, 2002, 22(2): 131-133 |
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| 作者姓名： | 刘立新 王迪浔 哈斯朝鲁 杜永成 |
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| 作者单位： | 1. 华中科技大学同济医学院,湖北,武汉,430030
2. 山西医科大学第一医院 |
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| 基金项目： | 国家自然科学基金重点项目资助 (39730 1 90 ) |
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| 摘 要： | 目的 探讨尼莫地平对过氧化氢 (H2 O2 )诱导人血管内皮细胞凋亡的影响。方法 在人脐静脉血管内皮细胞培养基中分别加入 H2 O2或 H2 O2 尼莫地平 ,培养 2 4 h,用流式细胞仪 PI标记法、原位末端脱氧核苷酸转移酶 (Td T)标记法 (TUNEL)和 HE染色法对细胞凋亡进行鉴定和检测。结果 H2 O2 能明显地诱导培养的人血管内皮细胞发生凋亡 ,尼莫地平可显著降低 H2 O2 诱导的内皮细胞凋亡率 (P<0 .0 1 )。结论 尼莫地平对 H2 O2 诱导的血管内皮细胞凋亡具有保护作用。
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| 关 键 词： | 尼莫地平 内皮 血管 细胞凋亡 过氧化氢 |
| 文章编号： | 1005-9202(2002)02-0131-03 |
| 修稿时间： | 2001-04-23 |
The effect of Nimodiping on vascular endothelial cell apoptosis induced by hydrogen peroxide |
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| LIU Li-xin,HA Sichaolu,Wang Dixun et al Tongji College of Huazhong Science and Technology University,Hubei Wuhan. The effect of Nimodiping on vascular endothelial cell apoptosis induced by hydrogen peroxide[J]. Chinese Journal of Gerontology, 2002, 22(2): 131-133 |
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| Authors: | LIU Li-xin HA Sichaolu Wang Dixun et al Tongji College of Huazhong Science Technology University Hubei Wuhan |
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| Affiliation: | LIU Li-xin,HA Sichaolu,Wang Dixun et al Tongji College of Huazhong Science and Technology University,Hubei Wuhan 430030 |
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| Abstract: | Objectives To study the effect of Nimodiping on vascular endothelial cell apoptosis induced by hydrogen peroxide (H_2O_2).Methods Cultured vascular endothelial cells (VECs) were treated with H_2O_2 or H_2O_2 Nimodiping for 24 hours. Flow cytometer, terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling (TUNEL), HE staining were employed to monitor cell apoptosis. Results H_2O_2 was able to induce apoptosis in human VECs. Nimodiping could depress human VECs apoptosis. Conclusions Nimopdiping could protect VECs apoptosis induced by H_2O_2. |
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| Keywords: | Nimodiping Endothelium Vascular Apoptosis Hydrogen peroxide |
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