| 大黄5个蒽醌类成分与大鼠血浆蛋白结合率的测定 |
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| 引用本文： | 冯素香,吴加,李建生,屈凌波,徐会平,杨冉,李建军. 大黄5个蒽醌类成分与大鼠血浆蛋白结合率的测定[J]. 中国现代应用药学, 2013, 30(1): 1-5 |
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| 作者姓名： | 冯素香 吴加 李建生 屈凌波 徐会平 杨冉 李建军 |
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| 作者单位： | 河南中医学院，郑州 450008；郑州大学，郑州 450001;河南中医学院，郑州 450008;河南中医学院，郑州 450008;郑州大学，郑州 450001；河南工业大学，郑州 450001;郑州大学，郑州 450001;郑州大学，郑州 450001;郑州大学，郑州 450001 |
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| 基金项目： | 国家自然科学基金资助项目(81274179)；河南省高校青年骨干教师资助项目(2009GGJS-065)；河南省教育自然科学研究计划项目(2010A360002) |
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| 摘 要： | 目的建立测定大黄苷元中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚5种成分与大鼠血浆蛋白结合率的方法。方法采用体外平衡透析法,模拟大黄苷元与血浆蛋白的结合过程,以固相萃取柱处理血浆样品,用高效液相色谱法测定大黄苷元中5种蒽醌类成分在透析袋内血浆中的浓度与透析袋外缓冲液中的浓度,计算血浆蛋白结合率。结果芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚提取回收率分别为68.6%,91.5%,78.6%,88.2%和71.4%,与大鼠体外血浆蛋白结合率分别为93.1%,91.3%,95.2%,87.5%和89.7%。结论大黄5种苷元与大鼠血浆蛋白结合率较高,且蛋白结合率与血药浓度无明显依赖性。
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| 关 键 词： | 大黄苷元 血浆蛋白结合率 平衡透析法 高效液相色谱法 |
| 收稿时间： | 2011-12-31 |
| 修稿时间： | 2012-07-11 |
Determination of the Binding Rate of Rat Plasma Protein with Five Anthraquinones in Rhubarb |
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| FENG Suxiang,WU Ji,LI Jiansheng,QU Lingbo,XU Huiping,YANG Ran and LI Jianjun. Determination of the Binding Rate of Rat Plasma Protein with Five Anthraquinones in Rhubarb[J]. The Chinese Journal of Modern Applied Pharmacy, 2013, 30(1): 1-5 |
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| Authors: | FENG Suxiang WU Ji LI Jiansheng QU Lingbo XU Huiping YANG Ran LI Jianjun |
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| Affiliation: | Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China; Zhengzhou University, Zhengzhou 450001, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Zhengzhou University, Zhengzhou 450001, China; Henan University of Technology, Zhengzhou 450001, China;Zhengzhou University, Zhengzhou 450001, China;Zhengzhou University, Zhengzhou 450001, China;Zhengzhou University, Zhengzhou 450001, China |
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| Abstract: | OBJECTIVE To develop a method for the simultaneous determination of the binding rate of plasma protein with aloe-emodin, rhein, emodin, chrysophanol and physcion. METHODS The equilibrium dialysis method was carried out to imitate the binding process between five anthraquinones and plasma protein. Plasma samples were treated with solid-phase extraction and the binding rate was determined by HPLC. RESULTS The extract recoveries of aloe-emodin, rhein, emodin, chrysophanol and physcion were 68.6%, 91.5%, 78.6%, 88.2% and 71.4%, the binding rates of plasma protein were 93.1%, 91.3%, 95.2%, 87.5% and 89.7%. CONCLUSION This method shows the high binding rate of five anthraquinones to plasma protein. The binding rates of five anthraquinones are not proportionally dependent on plasma concentration. |
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| Keywords: | rhubarb aglycone protein binding rate equilibrium dialysis HPLC |
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