| 应用抑制性消减杂交技术克隆EB病毒致人B淋巴细胞永生化的差异性表达基因 |
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| 引用本文： | 李茜,朱振宇,梁雪清,李满枝,黄必军.应用抑制性消减杂交技术克隆EB病毒致人B淋巴细胞永生化的差异性表达基因[J].中国组织工程研究与临床康复,2003,7(5):872-873. |
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| 作者姓名： | 李茜 朱振宇 梁雪清 李满枝 黄必军 |
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| 作者单位： | DepartmentofBiochemistrySunYat-senMedicalCollege，DepartmentofBiochemistrySunYat-senMedicalCollege，DepartmentofBiochemistrySunYat-senMedicalCollege，DepartmentofBiochemistrySunYat-senMedicalCollege，DepartmentofBiochemistrySunYat-senMedicalCollege SunYat-senUniversity，Guangzhou510089，GuangdongProvince，China，SunYat-senUniversity，Guangzhou510089，GuangdongProvince，China，SunYat-senUniversity，Guangzhou510089，GuangdongProvince，China，SunYat-senUniversity，Guangzhou510089，GuangdongProvince，China，SunYat-senUniversity，Guangzhou510089，GuangdongProvince，China |
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| 摘 要： | INTRODUCTIONEpstein－BarrVirus（EBvirus）wasfirstisolatedfromculturedBruitt＇lymphomabyEpstein，BarrandAchongin１９６４．EBvirusbelongstohumanherpesvirus．MostpeopleexperiencedEBvirusinfectionwithoutobvioussymptoms．EBvirusisinvolvedinalotofconditionssuchasinfectiousmononucleosissyndrom（IM），Burkitt＇lymphoma（BL），nasopharyngealcarcinoma（NPC），chronicactiveEBvirusin－fection（CAEBV），Hodgkin＇sdisease（HD），peripheralTcelllym－phoma，gastriccancerandimmunodefficientlym…
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Quick search of differentially expressed genes of EB virus resulting immortal human B cells by suppression subtractive hybridization |
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| Li Qian,Zhu Zhenyu,Liang Xueqing,Li Manzhi,Huang BijunLi Qian,Zhu Zhenyu,Liang Xueqing,Li Manzhi,Huang Bijun.Quick search of differentially expressed genes of EB virus resulting immortal human B cells by suppression subtractive hybridization[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2003,7(5):872-873. |
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| Authors: | Li Qian Zhu Zhenyu Liang Xueqing Li Manzhi Huang BijunLi Qian Zhu Zhenyu Liang Xueqing Li Manzhi Huang Bijun |
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| Institution: | Department of Biochemistry, Sun Yat-sen Medical College, Sun Yat-sen University, Guangzhou 510089, Guangdong Province, China |
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| Abstract: | AIM:To clone and identify genes differentially expressed in the EB virus transformed human B cell.METHODS:Suppression subtractive hybridization was used to construct the library which contains the differentiately expressed cDNAs in EB virus transformed human B cell.Then the isolated genes were cloned and sequenced and identifed by RT PCR.Nucleic acid homology searches were performed using the BLAST program.RESULTS:By this technique,4 differentiately expressed gene cDNA fragments of EB virus transformed human B cell were obtained.CONCLUSION:SSH is an effective method to isolate differentiately expressed genes. |
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| Keywords: | herpesvirus 4 human genetic techniques B lymphocytes |
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