液相色谱-串联质谱法检测水中微囊藻毒素LR和RR

目的:建立水中微囊藻毒素LR和RR的液相色谱-串联质谱检测方法。方法:水样经C18固相萃取小柱富集,甲醇洗脱,浓缩后,经XTerra MS C18(2.1×150 mm,5μm)柱进行色谱分离,采用多反应监测(MRM)模式对微囊藻毒素进行检测,外标法定量。结果:微囊藻毒素LR和RR的检出限分别为0.002μg/L和0.001μg/L,其加标回收率分别为84.6%～90.6%和86.6%～91.2%,相对标准偏差小于5.0%。结论:该方法用水量少,简便、快速、准确,适用于水中微囊藻毒素LR和RR的检测。

Determination of microcystin LR and RR in water by HPLC-MS/MS

Objective:A HPLC-MS/MS method was developed for the determination of Microcystin LR and RR in water.Methods: Sample concentration and purification were performed using C18 solid phase extraction cartridges,the analyte was eluted by methanol.The separation was performed on a XTerra MS C18 column(2.1×150 mm,5 μm).Multiple reaction monitoring(MRM) mode was applied in quantification of Microcystin LR and RR.Results: The limit of detection(LOD) was 0.002 μg/L and 0.001 μg/L for Microcystin LR and RR,respectively.The recoveries of microcystin LR and RR were 84.6%～90.6% and 86.6%～91.2%,respectively;with RSD less than 5.0%.Conclusion: This method is accurate and rapid,easy to operate for microcystin analysis in water.