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| 次声对视网膜微血管内皮细胞胞浆[Ca2+]i水平的影响 | |
| 引用本文： | 邱萍,李泱,温宁,姜勇,张作明,王士雯. 次声对视网膜微血管内皮细胞胞浆[Ca2+]i水平的影响[J]. 中国微循环, 2004, 8(2): 79-82 |
| 作者姓名： | 邱萍 李泱 温宁 姜勇 张作明 王士雯 |
| 作者单位： | 1. 100853,北京,解放军总医院老年心血管病研究所 2. 100853,北京,解放军总医院老年心血管病研究所口腔科 3. 第一军医大学病理生理教研室 4. 第四军医大学空军医学系航空临床教研室 |
| 基金项目： | 国家自然科学基金，军队重点项目，中国博士后科学基金 |
| 摘 要： | 目的通过检测次声暴露前后视网膜微血管内皮细胞(BREC)胞浆[Ca2 ]i水平的改变,对次声致大鼠血-视网膜屏障通透性改变的细胞内信号转导途径进行初步探讨.方法采用荧光探针Flu-3研究次声及BKca开放剂NS-1619干预下BREC胞浆[Ca2 ]i水平的改变.结果次声暴露导致细胞内钙库释放Ca2 ,同时观察到NS-1619外钙依赖性地增加[Ca2 ]i.结论次声引起[Ca2 ]i升高,通过细胞收缩成份引起内皮细胞主动收缩,从而达到对血-视网膜屏障的调节效应. |
| 关 键 词： | 次声 激光共聚焦显微镜 胞浆内游离钙离子浓度 |
| 文章编号： | 1007-8568(2004)02-0079-04 |
| 修稿时间： | 2003-09-30 |
The Study of Cytoplasmic Free Calcium Concentration of the Bovine Retinal Microvessel Endothelium Cells Under Infrasound Exposure |
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| QIU Ping,LI Yang,WEN Ning,et al.. The Study of Cytoplasmic Free Calcium Concentration of the Bovine Retinal Microvessel Endothelium Cells Under Infrasound Exposure[J]. Journal of Chinese Microcirculation, 2004, 8(2): 79-82 | |
| Authors: | QIU Ping LI Yang WEN Ning et al. |
| Affiliation: | QIU Ping,LI Yang,WEN Ning,et al.Geriatric Cardiology Institute,The General Hospital of PLA,Beijing100853,China |
| Abstract: | Objective To further understand the mechanism of the change that infrasound brings to BRB.Laser scanning confocal microscopy(LSCM)was used to detect the change of cytosolic free calcium[Ca 2 ]i in the bovine retinal microvessel endothelium cells(BREC)under infrasound exposure.Methods The change of[Ca 2 ]i under the infrasound exposure and NS-1619was studied by fluorescent indicator Flu-3.Results Pure exposure to infrasound can result in calcium release from the calcium store in the cell.Meanwhile,the increase of[Ca 2 ]i under NS-1619is also observed.Conclusions The infrasound may result in the increase of[Ca 2 ]i and through calcium-dependent interaction of endothelium,the adverse effect on BRB is achieved thereby. |
| Keywords: | Infrasound Laser scanning confocal microscopy Cytosoic free calcium concentration |
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