抗-戊型肝炎病毒单克隆抗体的研制及应用

目的研究戊型肝炎病毒（ＨＥＶ）颗粒，对戊型肝炎进行特异的诊断。方法采用基因工程表达的戊型肝炎病毒抗原作为免疫源，用ＥＬＩＳＡ间接法以纯化的人工合成多肽抗原检测。结果研制出４株稳定分泌抗ＨＥＶ的单克隆抗体（ＭｃＡｂ）５Ｂ１２、５Ｆ１、５Ｂ９和４Ｇ１０，所制备出的抗ＨＥＶ单克隆抗体与抗ＨＥＶ阳性血清可产生抑制反应；亦可与ＨＥＶ病毒颗粒特异地结合，形成在电镜下清晰可见的病毒抗体复合物。结论这种方法制备的ＭｃＡｂ特异性高，可用于病毒的检测和鉴定

Preparation of Monoclonal Antibodies against Hepatitis E and Its Application

Objective To study the particle of hepatitis E virus (HEV) and its significance in specific diagnosis for hepatitis E (HE). Methods Four strains of monoclonal antibodies (McAb) against HEV, 5B12, 5F1, 5B9 and 4G10, were prepared with HEV antigen (used as immunogen) expressed by genetic engineering and detected by indirect enzyme linked immunosorbent assay (ELISA) with purified synthetic polypeptide antigen. Results The monoclonal anti HEV prefared could produce inhibitory reaction with anti HEV positive serum and combined specifically with HEV particle forming virus antibody complex seen clearly under electron microscope. Conclusion McAb against HEV so prepared with high specificity can be used to detect and identify HEV.