| Regulative effect of P38MAPK on release of TNFα and NO from alveolar macrophages under endotoxin stimulation |
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| 引用本文： | 卢家凯 杨志焕 等. Regulative effect of P38MAPK on release of TNFα and NO from alveolar macrophages under endotoxin stimulation[J]. 中华创伤杂志(英文版), 2001, 4(2): 75-77 |
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| 作者姓名： | 卢家凯 杨志焕 等 |
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| 作者单位： | [1]ResearchInstituteofSurgery,DapingHospital,ThirdMilitaryMedicalUniversity,Chongqing400042,China [2]Resear,DapingHospital,ThirdMilitaryMedicalUniversity,Chongqing400042,China |
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| 摘 要： |
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| 关 键 词： | 内毒素 P38MAPK 肺泡巨噬细胞 TNFα NO |
Regulative effect of P38MAPK on release of TNFalpha and NO from alveolar macrophages under endotoxin stimulation. |
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| J Lu,Z Yang,J Jiang,Z Wang,P Zhu. Regulative effect of P38MAPK on release of TNFalpha and NO from alveolar macrophages under endotoxin stimulation.[J]. Chinese journal of traumatology, 2001, 4(2): 75-77 |
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| Authors: | J Lu Z Yang J Jiang Z Wang P Zhu |
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| Affiliation: | Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, 400042, China. |
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| Abstract: | OBJECTIVE: To evaluate activation of P38 mitogen-activated protein kinase (P38MAPK) in alveolar macrophage (AM), release of TNFalpha and NO from cells, and their relationship following lipopolysaccharide (LPS) stimulation. METHODS: AM was isolated from branch alveolar lavage fluid (BALF). The activation of P38MAPK was assayed by Western blot. SB203580, a specific inhibitor of P38MAPK, was used with gradient concentration to evaluate the regulative effect of P38MAPK on the release of TNFalpha and NO from AM. RESULTS: P38MAPK was activated by LPS (100 ng/ml) with peak activation at 30 minutes. The activation of P38MAPK was inhibited by SB203580. The secretion of TNFalpha and NO stimulated with LPS increased (P<0.01) and was inhibited by SB203580 significantly. CONCLUSIONS: The results indicate that P38MAPK is involved in the secreting process of TNFalpha and NO following LPS stimulation. P38MAPK may be an important site for controlling the secretion of both inflammatory mediators during lung inflammatory disorders. |
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