中药肾康灵干预阿霉素肾病大鼠血蛋白质质谱表达的实验研究

目的：应用血蛋白质组学筛选阿霉素肾病（AN）大鼠血清中差异表达的蛋白质,旨在蛋白质分子水平探索频复发性肾病综合征（FRNS）的诊断标志物及中药肾康灵治疗的作用靶点。方法：利用表面增强激光解析-电离飞行时间质谱（SELDI-TOF-MS）联合CM10蛋白质芯片技术检测各组大鼠血蛋白质组质谱的表达,采用PBSⅡ-C型蛋白质芯片阅读机自动收集数据,使用Ciphergen Proteinchip Software3.2.1和Biomarker Wizard分析软件筛选出各组大鼠血清差异表达的蛋白质。结果：在质荷比值（M/Z）2000~50000范围内,P〈0.05,芯片共捕获到30种差异蛋白质,质谱仪筛选出B组与A组比较的25种差异蛋白,C组与B组比较筛选出5种差异蛋白,其中3种差异蛋白在B、C、D、E组间差异有统计学意义,且从B组→C组→D、E组→A组呈递减趋势,这3种蛋白峰分别为8837.7、15013.0、15053.2。结论：SELDI-TOF-MS能有效分析阿霉素肾病大鼠血清蛋白质质谱,中药肾康灵干预治疗后蛋白质质谱表达差异有统计学意义。

The Empirical Study on Blood Proteomics of Rats with Adriamycin-Induced Nephropathy by Treament of Decoction Shenkangling

Objective：Purpose To analyze the blood proteomic patterns of rats with adriamycin-induced nephropathy by blood proteomics,for discussing the biomarker of Frequent Relapse Nephrotic Syndrome and target point of medication.Methods：Surface enhanced laser desorption/ionization-time of flight-mass spectrometry（SELDI-TOF-MS）CM10 protein clips was used to detect the blood proteomic patterns of every rat,PBS Ⅱ-C protein clips reading machinewas applied to collect data automaticly,proteins with differential expression between each group were analyzed by Ciphergen Proteinchip Software 3.2.1 and Biomarker Wizard software.Results：About 30 proteins with differential expression were detected in the mass charge ratio range of 2 000～50 000（P0.05）,there were 25 proteins between B group and A group,5 proteins between B group and C group,3 proteins decreased progressively from B group→C group→D、E group→A group,they were 8 837.7、15 013.0、15 053.2.Conclusion：The blood proteomic patterns of rats with adriamycin-induced nephropathy can be analyzed in effect by SELDI-TOF-MS,significantly changed by treament of decoction Shenkangling.