Increased trabecular bone mass in rats treated with human synthetic parathyroid hormone |
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| Affiliation: | 1. Division of Nephrology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, Taiwan;2. Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taiwan;3. Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taiwan;4. Division of Pulmonary and Critical Care, Department of Critical Care Medicine, Shuang Ho Hospital, Taipei Medical University, Taiwan;5. Division of Nephrology, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan;6. Department of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan;7. Department of Medical Research, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan;1. National School of Nursing and Obstetrics, National Autonomous University of Mexico, Mexico;2. Iztacala Faculty of Higher Studies, National Autonomous University of Mexico, Mexico;3. Rory Meyers College of Nursing, New York University, United States of America;4. National Institute of Cardiology, Mexico;5. Secretariat of Health, Mexico;6. Center for Health Systems Research, National Institute of Public Health, Mexico;1. Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, 101 Weill Hall, Ithaca, NY 14853, USA;2. Regional Bone Center, Helen Hayes Hospital, 55 N Route 9W, West Haverstraw, NY 10993, USA;3. Department of Pathology, Columbia University, 630 West 168th St., New York, NY 10025, USA;4. Department of Epidemiology, Columbia University, 722 West 168th St., New York, NY 10032, USA;5. Research Division, Hospital for Special Surgery, 515 East 71st St., New York, NY 10021, USA;6. Department of Medicine, Columbia University, 622 West 168th St., New York, NY 10032, USA |
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| Abstract: | Although PTH inhibits bone collagen synthesis acutely, prolonged administration of PTH increases trabecular bone mass. The objectives of this study were to develop a simple method to quantitate the anabolic response of cortical and trabecular bone, to determine if the trabecular bone response is at the expense of cortical bone, and to correlate the bone response with changes in serum calcium or phosphate. Subcutaneous injections of 2 to 16 μg/100 g body weight hPTH(1–34) were given daily to weanling rats for 12 days. The trabecular and cortical bone were manually separated from the distal femur, and calcium, hydroxyproline, and extracted dry weight were measured. Growth and renal function were not impaired. Serum calcium (range: 8.9–9.5 mg%) and serum phosphate (range: 7.7–8.5 mg%) did not differ significantly from control serum calcium, 9.1 mg%, or serum phosphate, 8.1 mg%. A dose-related anabolic response was observed in calcium, hydroxyproline, and dry weight of trabecular bone. At the highest dose of hPTH for 12 days, these values were increased above control by 26%, 33%, and 26%, respectively (P < .01). While cortical bone values increased over 12 days, only the dry weight increase was significant (P < .01). Our method showed an increase in trabecular bone mass after daily subcutaneous injections of hPTH (1–34) at doses that were neither toxic nor hypercalcemic. Although the absolute change of cortical bone mass was greater than that of trabecular bone, it was more variable and not statistically significant. Nevertheless, these results indicate that trabecular bone mass did not increase at the expense of cortical bone. |
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