高纯度GLA包合工艺研究Ⅱ——包合工艺的单因素、2因素试验

目的:考察尿素包合工艺各因素对纯度和产率的影响。方法:采用单因素试验考察原料的配比、包合温度、包合时间、对纯度的影响及3次包合后提取方法对产率的影响,采用2因素试验考察了不同配比和乙醇含量、包合次数和包合方法对产物的影响。结果和结论:单因素试验经方差分析和显著性测验说明原料的配比以1∶3∶8(油∶尿素∶95%乙醇)为最好,1次包合后纯度为30%左右,包合的最佳温度为-15～-20℃,包合时间以24h为好。2因素实验说明不同配比与乙醇含量最佳组合为配比1∶3∶8、乙醇为90%～95%,其纯度最高。包合的次数在3次以上时,纯度才可达到90%以上,且方法之间差异不显著。

Study on the encapsulation technique of high purity γ-linolenic acid, part 1-single factor, and two-factor tests for encapsulation process

OBJECTIVE: To study the effects of all factors during the process of urea encapsulation of gamma-linolenic acid on the purity and yield. METHOD: To observe the material proportions, time, temperature and purity using single-factor and two-factor tests. RESULT: Single-factor test showed that the optimal ratio of all materials (oil, urea and 95% ethanol) was 1:3:8. A 30% purity after single encapsulation process was obtained, at the best temperature range was - 15 degrees C-20 degrees C, for 24 hours. Two-factor test showed that the optimal ratio of oil, urea and ethanol was 1:3:8, where the concentration of ethanol was 90%-95%. The purity reached 90% or higher, with three-time encapsulation process. CONCLUSION: An optimized process was identified where material ratios, encapsulation time, temperature, and ethanol concentrations were determined using single-factor and two-factor tests.