Cell culture (Vero) derived whole virus (H5N1) vaccine based on wild-type virus strain induces cross-protective immune responses |
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Authors: | Kistner Otfried Howard M Keith Spruth Martin Wodal Walter Brühl Peter Gerencer Marijan Crowe Brian A Savidis-Dacho Helga Livey Ian Reiter Manfred Mayerhofer Ines Tauer Christa Grillberger Leopold Mundt Wolfgang Falkner Falko G Barrett P Noel |
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Affiliation: | Baxter AG, Biomedical Research Center, Uferstrasse 15, A-2304 Orth/Donau, Austria. |
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Abstract: | The rapid spread and the transmission to humans of avian influenza virus (H5N1) have induced world-wide fears of a new pandemic and raised concerns over the ability of standard influenza vaccine production methods to rapidly supply sufficient amounts of an effective vaccine. We report here on a robust and flexible strategy which uses wild-type virus grown in a continuous cell culture (Vero) system to produce an inactivated whole virus vaccine. Candidate vaccines based on clade 1 and clade 2 influenza H5N1 strains were developed and demonstrated to be highly immunogenic in animal models. The vaccines induce cross-neutralising antibodies, highly cross-reactive T-cell responses and are protective in a mouse challenge model not only against the homologous virus but also against other H5N1 strains, including those from another clade. These data indicate that cell culture-grown whole virus vaccines, based on the wild-type virus, allow the rapid high yield production of a candidate pandemic vaccine. |
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