Characterization of specific binding sites for high-density lipoproteins on rat hepatocytes: Effects of estradiol and testosterone |
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| Authors: | T G Vishnyakova A V Bocharov I N Baranova V S Repin |
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| Institution: | (1) Laboratory of Cell and Tissue Cultures, Institute of Experimental Cardiology, Cardiology Research Center, Russian Academy of Medical Sciences, Moscow |
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| Abstract: | Two types of binding sites for high-density lipoproteins (HDL): P1 and P2 Kdl=20 and Kd2=2.5μg/ml, N1=130 and N2=35 ng/mg cell protein) are identified on the surface of rat hepatocytes. Conditions for predominant determination of P2 are created by employing radiolabeled lipoproteins (125I-HDL) with a high specific activity (1000 dpm) and the differences in the kinetics of the P1-and P2-125I-HDL complex formation. P2 predominate on hepatocytes from females. The addition of estradiol to a culture of hepatocytes from males increases the content
of P2, while the addition of testosterone to hepatocytes from females decreases the content of P2 to the levels determined in males.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 12, pp. 629–634, December, 1996 |
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| Keywords: | hepatocytes cell culture high-density lipoproteins specific binding androgens estrogens |
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