肺癌患者GSTT1多态性分布及其与15号染色体畸变的关系

 目的分析肺癌患者与对照GSTT1基因型多态性分布差异,探讨GSTT1基因型多态性对15号染色体稳定性的影响以及该染色体与肺癌发生之间的关联。方法利用PCR技术分析了61例肺癌患者和46例对照的GSTT1基因型分布,以荧光原位杂交(FISH)技术分析了不同GSTT1基因型的14例肺癌患者和18例对照淋巴细胞15号染色体结构和数目畸变。结果GSTT1+基因型(+/+和+/0)和GSTT1缺失基因型(0/0)在肺癌患者和对照之间的分布无显著性差异;肺癌组GSTT1+基因型15号染色体结构畸变率显著高于对照组GSTT1+和GSTT10/0型(P<0.05),但肺癌组0/0基因型未表现这种关系;15号染色体非整倍体发生率在各组和各基因型之间未表现显著差异。结论GSTT1基因型多态性与肺癌风险、15号染色体的稳定性无显著相关,15号染色体结构畸变是肺癌的风险因素之一。

The Relationship of GSTT1 Polymorphism and Chromosome 15 Aberration in Lung Cancer Patients

Objective The research analyzed the difference of GSTT1 genotype polymorphism between lung cancer cases and controls,evaluated the association of the genetic polymorphism and aberration of chromosome 15 with lung cancer.Methods GSTT1 genotypes from 61 lung cancer cases and 46 controls were determined by PCR and structural and number aberration of chromosome 15 were detected by FISH.Results There was no any differences of distribution of GSTT1 positive(+/+ and +/0) and GSTT1 null(0/0) genotypes between case and control groups.The frequency of chromosome structural aberration of lung cancer case with GSTT1 positive genotype was significantly higher than control with both genotypes(P< 0.05 ), no same relationship was found in those of lung cancer cases with GSTT1 null genotype.The experiment didn't show any significant differences on the incidence of chromosome 15 aneuploidy in any sampling group with any GSTT1 genotype.Conclusion There was no significant association between GSTT1 genotype with lung cancer and/or the stability of chromosome 15.Structural aberration of chromosome 15 may be one of the risk factor of lung caner.