头皮针对脑缺血大鼠模型碱性成纤维细胞生长因子的影响

目的 :研究头皮针对脑缺血大鼠模型碱性成纤维细胞生长因子的影响 ,并与电针组比较。方法 :线栓法制备大鼠脑缺血模型 ,甲醛固定脑组织切片 ,运用免疫组化方法检测各组碱性成纤维细胞生长因子的变化情况。结果 :术后 6hr与术后 1 5天组内神经功能评分比较 ,模型组无明显差异 (P >0 .0 5) ,电针组与针刺组有显著性差异 (P <0 .0 1 )。组间 1 5天时比较 ,电针组与针刺组无明显差异 (P >0 .0 5) ,两组与模型组比较均有显著性差异 (P <0 .0 1 )。在第 1 5天时碱性成纤维细胞生长因子免疫组化染色比较 ,电针组与模型组有明显差异 (P <0 .0 5) ,针刺组与模型组有显著性差异 (P <0 .0 1 ) ,针刺组与电针组有明显差异 (P <0 .0 5)。结论 :头皮针可促进碱性成纤维细胞生长因子产生并延长碱性成纤维细胞生长因子产生时限 ,这可能是头皮针减轻脑缺血损伤并促使肢体功能恢复的机理之一

Effect of Scalp Acupuncture Therapy on Cerebral Basic Fibroblast Growth Factor Expression in Cerebral Ischemia Rats

Objective: To study the effect of scalp acupuncture on cerebral basic fibroblast growth factor (bFGF) expression in cerebral ischemia model rats. Methods: A total of 50 Wistar rats were randomized into control group, sham operation group, model group, electroacupuncture (EA) group and hand acupuncture group, with 10 cases being in each group. Cerebral ischemia model was established by obstructing blood flow of the right middle cerebral artery with a nylon thread embolus. Two acupuncture needles were inserted into "Baihui" (GV 20) and "Qubin" (GB 7) separately to advance oppositely along the scalp, with the scalp points stimulated with EA or hand manipulation. The treatment was given once daily, continuously for 15 days. The brain tissue sample was fixed with formaldehyde, sliced up, and then stained with immunohistochemical technique for observing changes of bFGF immuno reaction (IR) positive neurons of the cerebral tissue. Results: Auto comparison between 6 hr and 15 d after operation in neurological defect score showed no significant difference in model group ( P >0.05), but in EA group and hand acupuncture group this neurological defect score decreased apparently after treatment ( P <0.01). There were notable differences between EA group and model group, and between hand acupuncture and model group in the neurological score ( P <0.01); while no distinct difference was found between EA group and hand acupuncture group ( P >0.05), suggesting a favorable effect of acupuncture in improving cerebral ischemia induced neurological functional disturbance. Results of immunohistochemical staining displayed that bFGF expression in model group appeared in 3 animals (no bFGF expression in control and sham operation groups); the density of bFGF IR in both EA group and hand acupuncture group increased strikingly compared with model group ( P <0.05～0.01); and the effect of hand acupuncture group was superior to that of EA group ( P < 0.05 ). It suggests that acupuncture induced up regulation of bFGF expression maybe contribute to its therapeutic effect in bettering cerebral ischemia and promoting the recovery of the limbs after stroke. Conclusion: Scalp acupuncture can up regulate the expression of bFGF in the brain tissues of the cerebral ischemia rats.