经心内膜心肌内移植猪自体骨髓间充质干细胞治疗心肌梗死的实验方法

目的:探讨经皮经心内膜心肌注射移植猪自体骨髓间充质干细胞(bone marrow mesenchymal stem cells，BMSCs)的治疗心肌梗死(MI)实验方法的可行性和安全性。方法: 选5只雌性健康小型猪通过股动脉入路采用冠脉球囊封堵冠状动脉左前降支60 min建立MI模型。以超声确定MI后两周，采用心肌注射器经心内膜向心肌内注入1×108个小型猪自体红色荧光染料Dil标记的BMSCs，并观察心电图(ECG)的变化。移植BMSCs 8周后，取心肌组织行病理切片，选取含有移植细胞的切片行免疫荧光染色。结果: 4只小型猪成功地建立MI模型（1只因术中室颤死亡）并完成经心内膜的细胞移植，注射过程可出现一过性室性早搏以及短阵室速。通过心肌免疫荧光染色证实，移植的BMSCs已在宿主心肌内存活。结论: 使用心肌注射器经心内膜心肌移植自体BMSCs创伤小，方法可行，具有临床应用的前景。

Method of catheter-based transendocardial delivery of bone marrow mesenchymal stem cells into swine-infarcted heart

AIM: To study the feasibility and safety of myocardial syringe for transendocardial delivery of autologous bone marrow mesenchymal stem cell(MSCs) into swine-infarcted hearts.METHODS: The left anterior descending coronary artery of swine was occluded for 60 min by coronary balloon to establish the myocardial infarction model.After 2 weeks,autologous bone marrow MSCs were transplanted into myocardium using myocardial syringe.Eight weeks later,animal hearts were harvested for pathological examination by immunofluorescence.RESULTS: Four swine models were successfully established and one died due to ventricular fibrillation during coronary artery occlusion.Left ventricular contrast imaging localized the endocardium-injected points.Immunofluorescence further demonstrated the survival of the injected cells in the host’ s heart.CONCLUSION: Catheter-based transendocardial delivery of bone marrow MSCs into myocardium is feasible and safe.