外源性精胺对人脐静脉内皮细胞的损伤作用及机制研究

目的： 观察外源性精胺(Sp)对传代培养的人脐静脉内皮细胞(HUVECs)的作用，并初步探讨其可能机制。方法：采用传代培养的方法，观察不同浓度精胺(50 μmol/L-5 mmol/L)对HUVECs作用2 h、4 h的影响。观察指标：(1)倒置显微镜下观察HUVECs形态学变化；(2)透射电镜观察HUVECs超微结构改变；(3)细胞活力测定； (4)MDA含量测定；(5)SOD活性测定。结果：对HUVECs影响的各项观察指标，50 μmol/L Sp与正常对照组比较，无显著差异(P>0.05)；200 μmol/L、1 mmol/L、5 mmol/L Sp对HUVECs有损伤作用，作用4 h的细胞损伤较2 h重，其特点为细胞活力降低，超微结构损伤，MDA含量升高，SOD活性降低。 结论：Sp呈浓度依赖性引起HUVECs损伤，其作用机制可能与氧自由基大量产生导致的细胞膜脂质过氧化有关。

Damaging effects of exogenous spermine on HUVECs and its possible mechanism

AIM: To study the effects of exogenous spermine on human umbilical veins endotheliocytes (HUVECs) and to explore its possible mechanism. METHODS: The serial subculture of HUVECs was used to investigate the effect of exogenous spermine with different concentrations (50 μmol/L-5 mmol/L) on HUVECs in different times (2 h, 4 h). The morphological changes of HUVECs (by inverted microscope and electron microscope), the cell viability, the level of MDA and activity of SOD were observed. RESULTS: Compared to normal control group, no change of all index detected in the group with spermine (50 μmol/L) was observed (P>0.05). Spermine injured HUVECs in a concentration-dependent manner. After adding spermine for 2 h and 4 h, it was observed that cellular injury in 4 h group was more serious than that in 2 h group. The injury of HUVECs caused by exogenous spermine was characterized by decrease in cellular viability and activity of SOD, ultrastructural injury, increase in MDA level. CONCLUSION: Exogenous spermine induces the injury of HUVECs in concentration and time-dependent manners. Its mechanisms may be related to lipid peroxidation induced by increase in the production of oxygen free radical.