| HPLC测定超微与常规戊己丸中芍药苷含量的比较 |
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| 引用本文： | 李荣东,史俊,蔡光先.HPLC测定超微与常规戊己丸中芍药苷含量的比较[J].亚太传统医药,2010,6(8):23-24. |
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| 作者姓名： | 李荣东 史俊 蔡光先 |
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| 作者单位： | 1. 湖南中医药大学,湖南长沙,410208
2. 湖南省中医药研究院,湖南长沙,410000 |
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| 摘 要： | 目的:建立测定戊己丸中芍药苷含量的HPLC方法,并对超微戊己丸和常规戊己丸中芍药苷的含量进行比较。方法:色谱柱为大连依利特公司HypersilC18柱(4.6mm×250mm,5μm),流动相:乙腈—水(14:86),流速:1mL/min,检测波长:230nm。结果:芍药苷的理论塔板数均不低于3000。芍药苷回归方程:Y=13.614X-1.4897,r=0.9999,线性范围10.2～102μg.mL-1。超微戊己丸中芍药苷平均回收率为99.05%,RSD为0.4%;常规戊己丸中芍药苷平均回收率为97.10%,RSD为1.6%。结论:超微戊己丸中芍药苷的含量明显高于常规戊己丸。
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| 关 键 词： | HPLC 芍药苷 戊己丸 |
The Comparison of the Content of Paeoniflorin in Ultramicro and General Wuji Pills |
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| Li Rongdong,Shi Jun,Cai Guangxian.The Comparison of the Content of Paeoniflorin in Ultramicro and General Wuji Pills[J].Asia-Pacific Traditional Medicine,2010,6(8):23-24. |
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| Authors: | Li Rongdong Shi Jun Cai Guangxian |
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| Institution: | 1.Hunan University of Traditional Chinese Medicine, Changsha 410208, China; 2.Research Institution of Hunan Province,Changsha 410000,China) |
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| Abstract: | Objective ：To establish a HPLC method for the content of Paeoniflorinin Wnji Pills by HPLC,and compare it between Ultramicro Wuji Pills and General Wuji Pills.Methods：Chromatographic colmn：hypersil BDS C18 （4.6ram ×250mm,5μm）, mobile pbase：acetonitrile-water （14：86）,flow rate lmIJmin, wavelength 230nm. Results：The nmber of theoretical plates calculated by Paeoniflorin peak was not below 3000. The regression equation of Ultramicro Paeoniflorinin was Y= Y=13.614X-1.4897 （r=0.9999）and the linear range was 10.2-102？g.mL-1. The average recovery of Ultramicro Paeoniflorin was 99.05%（11=5）, RSD0.4% ;The average recovery of General Paeoniflorin was 97.10%（n=5）, RSD1. 6%.Conclusion：The contents of Paeoniflorin in Ultramicro Wuji Pills are higher than General Wuji Pills. |
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| Keywords: | HPLC |
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