载氧脂质微泡体外释氧效果

目的评价载氧脂质微泡在缺氧溶液及离体静脉血中的释氧能力,观察超声辐照对微泡释氧量的影响。方法制备载氧脂质微泡,放置不同时间后(0.5、3.0、24.0h),检测微泡在极度缺氧溶液(0.4mg/L)中释氧量的改变;观察超声辐照条件下中度缺氧溶液溶解氧浓度(DO)值的变化;将载氧脂质微泡与离体静脉血混合后,检测静脉血p(O2)值的变化。结果载氧脂质微泡放置不同时间(0.5、3.0、24.0h)后释氧能力无明显改变(P>0.05);超声辐照能显著增加微泡的释氧量(P<0.05);载氧脂质微泡可有效增加离体静脉血p(O2)(P<0.05)。结论载氧脂质微泡能稳定载氧达24.0h;超声辐照下,微泡的释氧量明显增加;载氧脂质微泡能显著增加离体血p(O2),有望成为一种血浆代用品。

Oxygen release from lipid-coated oxygen microbubbles in vitro

Objective To assess the oxygen release kinetics of lipid-coated oxygen microbubbles in the hypoxic condition (normal saline and venous blood in vitro) and the effect of ultrasound (US) on oxygen release from these microbubbles. Methods After the preparation of lipid-coated oxygen microbubbles, a series of experiments were carried out to investigate the oxygen release kinetics in severe hypoxic (0.4 mg/L) solutions over different time (0.5, 3.0, 24.0 h), to observe the changes of oxygen concentration in moderate hypoxic (4.0 mg/L) solutions with US, and to test the blood oxygenation of venous blood in vitro after mixing with microbubble fluids. Results The oxygen release stayed the same level until 24.0 h (P>0.05). The oxygen concentration could be enhanced after insonation with US (P<0.05). The microbubbles improved p(O₂) of venous blood (P<0.05) in vitro effectively. Conclusion The self-made lipid-coated oxygen microbubbles has good capacity of loading oxygen for 24.0 h, release more oxygen with US, and the p(O₂) of venous blood in vitro remarkably increase, which may become a kind of blood substitute.