加味温胆汤对抑郁模型大鼠学习记忆能力及海马cAMP-PKA途径的影响

目的:探讨加味温胆汤对抑郁模型大鼠学习记忆能力及海马信号转导cAMP-PKA途径的影响。方法:32只SD大鼠随机分为正常组、模型组、盐酸氟西汀组及加味温胆汤组,每组8只,应用孤养加慢性轻度不可预见性应激方法造模,采用Morris水迷宫实验测试大鼠空间学习记忆能力、放射免疫法测定海马cAMP含量,以及免疫组化SABC法测定海马区PKA蛋白表达水平。结果:模型组大鼠学习记忆能力显著下降(P<0.01),海马cAMP含量增加(P<0.05)、PKA表达显著减少(P<0.01)。中、西药组与模型组比较,学习记忆能力增强(P<0.05),cAMP含量下降(P<0.05),PKA蛋白表达显著上升(P<0.05)。结论:加味温胆汤可增强抑郁症大鼠学习与记忆能力,其影响机制可能与海马信号传导cAMP-PKA途径有关。

Effects of Jiawei Wendan decoction on learning and memory and cAMP-PKA pathway of hippocampal signal transduction in rats model of depression

Objective:To observe effects of Jiawei Wendan decoction on learning and memory and cAMP -PKA pathway of hippocampal signal transduction in depressive rat models. Method: Depressive rat model was established by separation and chronic unpredictable mild stress. Thirty-two SD rats were selected and randomly divided into normal group, model group, Prozac group and Jiawei Wendan decoction group with 8 rats in each group. All rats were tested for spatial learning and memory in Morris water maze. Contents of cAMP were detected by radio - immunity assay,and the expression of PKA in CA3 region of the hippocampus was assayed by using immunohistochemical method. Result: Scores of spatial learning and memory lowered remarkably in model group, contents of cAMP increased and expressions of PKA remarkably reduced in hippocampus of model rats (P<0.01). Compared with model group, scores of spatial learning and memory increased obviously(P<0.05), contents of cAMP reduced (P<0.05) and expressions of PKA increased in drugs groups (P<0.05).Conclusion: These results indicate that Jiawei Wendan decoction can enhance the hippocampus-dependent spatial learning and memory function, and cAMP-PKA pathway of hippocampal signal transduction may participate in the mechanism of enhanced performance.