成人特发性血小板减少性紫癜淋巴细胞亚群研究

目的研究淋巴细胞亚群在成人特发性血小板减少性紫癜（ITP）治疗前后及正常对照人群中的表达变化,探讨其在ITP发病机制中的作用及临床意义。方法应用流式细胞术检测23例ITP患者治疗前、后及23例正常对照者外周血淋巴细胞亚群的表达水平,包括CD 3＋、CD 3＋CD 4＋、CD 3＋CD 8＋、CD 19＋淋巴细胞百分比及CD 3＋CD4＋/CD 3＋CD 8＋比值。结果 ITP患者治疗前CD 3＋T淋巴细胞百分比、CD 3＋CD 4＋T淋巴细胞百分比及CD 3＋CD4＋/CD 3＋CD 8＋比值均较正常对照组及ITP治疗后组减低（P〈0.05）,CD 3＋CD 8＋T淋巴细胞百分比及CD 19＋B淋巴细胞百分比较正常对照组及ITP治疗后组升高（P〈0.05）,正常对照组与治疗后ITP组四项指标无明显统计学差异（P〈0.05）。结论 T、B淋巴细胞亚群异常参与了ITP的发病,对其检测可能对ITP的诊断及治疗有帮助。

Study on Changes and Clinical Significance of Lymphocyte Subtypes in Adult Patients with Idiopathic Thrombocytopenic Purpura

Objective To study the changes in lymphocyte subsets in patients with idiopathic thrombocytopenic purpura(ITP) before and after therapy and healthy controls,and then to explore the clinical significance of the related factors envolved in pathlgenesis of ITP.Methods The percentages of lymphocyte subsets in the perpheral blood of 23 patients with ITP and 23 heaithy controls were detected by flow cytometry,including CD 3+,CD 3+CD 4+,CD 3+CD 8+,CD 19+ lymphocytes and CD 3+CD 4+/CD 3+CD 8+.Results The percentages of CD 3+T lymphocyte,CD 4+T lymphocyte and the ratio of CD 4+/CD 8+ in patients with ITP were obviously lower than those in healthy controls and ITP after immunosuppressive therapy(P<0.05),but the percentages of CD 3+ CD 8+T lymphocyte and CD 19+B lymphocyte was much higher(P<0.05) than those in heaithy controls and ITP afrer immunosuppressive therapy.There were no significant difference between the healthy controls and ITP afrer therapy(P<0.05).Conclusions The aberrant changes in T and B lymphocyte subsets are involved in the pathogenesis of ITP,and detection of those changes might be helpful in diagnosis and treatment of ITP.