Transwell小室共培养条件下RPE促进Müiler细胞的增殖和迁移

目的：观察在体外共培养系统中RPE对Müiler细胞的影响。方法：Transwell小室共培养RPE和Müiler细胞，MTT法、细胞计数法，测定Müiler增殖和迁移的情况。结果：Müiler细胞增殖的实验：Müiler细胞的增殖。除了3h与6h，24h与48h之外，在其他各时间点之间差别有统计学意义。Müiler细胞与RPE共培养组和Müiler细胞单独培养组两组之间差别有统计学意义。Müiler细胞迁移的实验；Müiler细胞迁移，除了3h和6h之外，在其他各时间点之间差别有统计学意义。Müiler细胞与RPE共培养组和Müiler细胞单独培养组两组之间差别有统计学意义。在析因设计实验中，与RPE共培养、缺氧条件，这两个因素都可以分别作为独立的因素促进Müiler细胞的增殖和迁移，而两者不存在协同作用。结论：正常和缺氧条件下RPE促进Müiler的增殖、迁移，随共培养时间的延长RPE促进Müiler增殖、迁移的作用加强。缺氧条件下RPE与Müiler细胞间的相互作用在视网膜增殖性疾病中起到了重要的作用。

Promotion of proliferation and migration of Müiler cells by RPE cells in a co-culture system

AIM： To investigate the role of retinal pigment epithelium （RPE） in the growth of Müiler cells using a coculture system in vitro.
METHODS： Müiler cells were cocultured with RPE cells under both normoxic and hypoxic conditions in Transwell chamber culture system. Müiler cell proliferation was evaluated by MTT assay. The number of cells which migrate through micropores and stay on the outer bottom side of insert systems were observed and counted.
RESULTS： The activities of proliferation and migration of Müiler cells when cocultured with RPE cells were significantly higher than those of the Müiler cells when cultured alone at all time points under both normoxic and hypoxic conditions. However, for both the coculture and control groups, there is no significant difference between the measurements at 3 and 6 hours.
CONCLUSION： Evidence suggests that RPE, when cocultured with Müiler cells, can stimulate migration and proliferation of Müiler cells under both hypoxic and normoxic conditions in a time-dependent manner; however, there is no evidence to support the synergetic interaction of RPE and Müiler cells co-cultured under hypoxic conditions.