SD大鼠Mller细胞的原代培养

目的：原代分离培养SD大鼠Müller细胞,在实验室建立Müller细胞株。方法：采用改良的酶消化法培养新生大鼠的视网膜Müller细胞,免疫组化法进行鉴定。结果：原代培养的Müller细胞胞体狭长,胞浆丰富,经过3～5次传代后逐渐变得胞体宽大,出现微丝和突起。免疫组化显示,95%以上的细胞谷氨酰胺合成酶染色阳性。结论：改良的酶消化法是培养视网膜Müller细胞的简单有效的方法。

Primary culture of Sprague-Dawley rat moiler cells

AIM： To culture müller cell and established cell strain in our laboratory.
METHODS： The retina was obtained from neonate rat and müller cells were isolated by Modified enzyme digestion. The cultured müller cell was identified by immuocytochemistry, and was observed under inverse phase microscope.
RESULTS： The cultured müller cell had large cell body and abundant kytoplasm. The body became wider and microfilament and cytodendrite appeared after 3-5 passage. More than 95% of the cells were positive stained for the glutamine synthetase.
CONCLUSION： The modified enzyme digestion method is a simple and effective way for culturing müller cells.