合肥地区506例子宫颈人乳头瘤病毒感染情况分析

目的探讨合肥地区女性子宫颈感染人乳头瘤病毒(HPV)的基因型分布和感染率情况。方法采用核酸分子快速导流杂交基因芯片技术(HybriMax)对妇科阴道镜门诊506例患者进行HPV21分型检测,阴道镜下宫颈多点活检并经病理证实病变程度。结果①HPV总的阳性率为46.6%(236/506),宫颈病变程度加重,HPV阳性率逐渐升高(25.0%～100.0%)。最常见的高危感染亚型为HPV16(50.0%)和52(26.7%),其次是33(11.4%),58(8.5%),18(6.4%);②HPV阳性患者单一感染和多重感染率分别为62.7%(148/236)和37.3%(88/236),多重感染中主要以二重感染为主86.4%(76/88),尤其以16和52型为主。CIN2-3组多重感染比例较高,与正常和炎症组、CIN1组和宫颈癌组比较有统计学意义(P<0.05),宫颈癌患者则以单一感染多见。结论合肥地区女性子宫颈感染HPV最常见的亚型为16、52、33、58和18。二重感染尤其是HPV16/52联合其他亚型与宫颈CIN2-3密切相关,而与宫颈癌关系不大。

Analysis of the prevalence of human papillomaviruses infection in the cervical of 506 women in Hefei

Objective To determine the infection rate of HPV and genotypes distribution in Chinese women of Hefei.Methods A total of 506 colposcopy outpatient women were checked for 21 HPV DNA types by DNA Flow-through Hybridization and Gene Chip,and cervical biopsies were used for pathological diagnosis.Results ① The overall HPV prevalence was 46.6%（236/506）,with cervical lesion severity,HPV positive rate were increased （25.0% ～100.0%）.The most common high risk types were HPV16（50.0%）and 52（26.7%）,followed by 33 （11.4%）,58（8.5%）and 18（6.4%）;② Single and multiple infection rate were 62.7%（148/236）and 37.3%（88/236）,respectively.Dual infection was main type of multiple infection,the most common models was HPV16/52 combined with other types.CIN2-3 multiple infection rate was higher compared to negative or cervix,CIN1 and cervical cancer（P0.05）.Single infection was common in cervical cancer.Conclusion HPV16,52,18,33 and 58 are the most prevalence high-risk subtypes in Hefei.Double infection especially HPV16/52 in conjunction with other subtypes is associated with CIN2-3,but not cervical cancer.