右美托咪定对骨巨细胞瘤细胞增殖、侵袭及迁移的影响

目的探究右美托咪定(dexmedetomidine,DEX)对骨巨细胞瘤细胞增殖、侵袭及迁移的影响.方法将细胞分Control、DEX 0.01、0.1及1μmol/L组.BrdU检测细胞增殖,transwell检测细胞侵袭,划痕实验检测细胞迁移,免疫印迹检测Ki67、PCNA、Bcl-2、VEGF、MMP-2、MMP-9、NF-κB p65及其下游蛋白表达水平、p38 MAPK表达及其下游蛋白磷酸化比值.结果与Control组比较,DEX 0.1、1μmol/L组BrdU阳性细胞百分比、侵袭细胞数、划痕闭合率和Ki67、PCNA、Bcl-2、VEGF、MMP-2、MMP-9表达水平显著降低,抑制p38 MAPK、NF-κB信号通路的激活.结论DEX通过调控p38 MAPK及NF-κB表达抑制骨巨细胞瘤细胞增殖、侵袭及迁移.

Effects of Dexmedetomidine on Proliferation,Invasion and Migration of Bone Giant Cell Tumor Cells

Objective To investigate the effects of dexmedetomidine on proliferation,invasion and migration of bone giant cell tumor cells.Methods The cells were divided into control,DEX 0.01,0.1 group and1μmol/Lgroup.Cell proliferation was detected by BrdU;cell invasion by tr-answell,cell migration by scratching experiment,and the expression of Ki67,PCNA,Bcl-2,VEGF,MMP-2,MMP-9,NF-κB p65 and their downstream proteins p38 MAPK and its down-stream protein phosphorylation ratio by Western blotting.Results Compared with the control group,the percentage of positive BrdU cells,the number of invasive cells,the scratch closure rate and the expression levels of Ki67,PCNA,Bcl-2,VEGF,MMP-2 and MMP-9 in DEX 0.1 and 1μmol/L groups were significantly decreased,which inhibited the activation of p38 MAPK and NF-κB p65 signaling pathways.Conclusion DEX inhibits the proliferation,invasion and migration of giant cell tumor cells by regulating the expression of p38 MAPK and NF-κB p65.