血尿淀粉酶、CRP、Card9蛋白在AP患者中的变化及其意义

目的探讨血尿淀粉酶、C反应蛋白(CRP)、胱天蛋白酶募集域蛋白9(Card9)在急性胰腺炎(AP)患者中的表达及其意义。方法选取本院2014年6月至2016年12月确诊的128例AP患者(AP组)、年龄及性别相匹配的健康自愿者64例(对照组),采用全自动生化法检测两组的血尿淀粉酶、血清CRP水平,采用酶联免疫吸附测定(ELISA)法检测两组外周血单核细胞中Card9蛋白表达水平,并根据AP患者病情分为轻症AP、重症AP进行分层分析。结果AP组患者的血尿淀粉酶、CRP、Card9蛋白水平均显著的高于对照组,差异具有统计学意义(P<0.05);重症AP组患者的血尿淀粉酶、CRP、Card9蛋白水平均显著的高于轻症AP组患者,差异具有统计学意义(P<0.05);AP组患者的血尿淀粉酶、CRP、Card9蛋白水平与Ranson评分均呈显著的正相关关系(P<0.05)。结论AP患者血尿淀粉酶、CRP、Card9蛋白水平升高显著,并且与病情的严重程度具有一定的相关性。

Changes and significance of hematuria,amylase,CRP and Card9 protein in patients with acute pancreatitis

Objective To investigate the expression and significance of hematuria,CRP and Card9 in patients with AP.Methods A total of 128 AP patients(AP group)confirmed to be diagnosed from June 2014 to December 2016 in our hospital and 64 healthy volunteers(control group)matched with age and gender were recruited.The levels of hematuria amylase and serum CRP were detected by automatic biochemical method.The expression of Card9 protein in peripheral blood mononuclear cells was detected by ELISA.According to the condition of AP patients,they were divided into mild AP and severe AP for stratified analysis.Results The levels of hematuria amylase,CRP and Card9 protein in the AP group were significantly higher than those in the control group,and the difference was statistically significant(P<0.05).The levels of hematuria amylase,CRP,and Card9 protein in the severe AP group were significantly higher than those in the mild AP group(P<0.05).The serum urinary amylase,CRP,and Card9 protein levels in the AP group had a significant positive correlation with the Ranson score(P<0.05).Conclusion The levels of hematuria amylase,CRP,and Card9 protein in AP patients increased significantly,and there was a certain correlation with the severity of the disease.