CDKN2B-AS1靶向miR-411-3p调节乳腺癌细胞的生物学行为

目的探究CDKN2B-AS1靶向miR-411-3p对乳腺癌MCF-7细胞的生物学行为的影响.方法将sh-CDKN2B-AS1、miR-411 inhibitor转染于MCF-7,再共同转染于MCF-7.检测各组细胞增殖、迁移、侵袭及蛋白表达.MCF-7及转染sh-CDKN2B-AS1的MCF-7接种于小鼠体内,为Ctrl及sh-CDK组,检测肿瘤组织CDKN2B-AS1、miR-411-3p、Ki67及VEGF.结果miR-411-3p是CDKN2B-AS1的靶向位点,相比于Ctrl组,sh-CDKN2B-AS1组CDKN2B-AS1、VEGF及P38表达量降低;miR-411-3p,ki67、HIF-1 a,Caspase-3表达量上升,细胞增殖数、侵袭数及迁移率降低(P均<0.05).与Ctrl组比较,sh-CDKN2B-AS1组肿瘤质量降低,肿瘤组织CDKN2B-AS1、ki67、VEGF表达量降低,miR-411-3p表达量上升(P均<0.05).结论抑制CDKN2B-AS1表达可靶向提升miR-411-3p水平,从而抑制乳腺癌细胞增殖、迁移、侵袭及移植瘤生长.

CDKN2B-AS1 Targets miR-411-3p to Regulate Biological Behavior of Breast Cancer Cells

Objective To investigate the effect of CDKN2B-AS1 targeting miR-411-3p on the biological behavior of breast cancer MCF-7 cells and transplanted tumors.Methods Sh-CDKN2B-AS1 and miR-411 inhibitor were transfected into MCF-7,and then co-transfected into MCF-7.Cell proliferation,migration,invasion and protein expression were detected in each group.MCF-7 and MCF-7 transfected with sh-CDKN2B-AS1 were inoculated in mice in the Ctrl and sh-CDK groups.CDKN2B-AS1,miR-411-3p,Ki67,and VEGF were detected in tumor tissues.Results miR-411-3p is the target site of CDKN2B-AS1.Compared with the Ctrl group,the expression levels of CDKN2B-AS1,VEGF and P38 in the sh-CDKN2B-AS1 group are reduced,Caspase-3 expres-sion increased,the number of cell proliferation,invasion and migration decreased(allP<0.05).Compared with the Ctrl group,the tumor mass of the sh-CDKN2B-AS1 group decreased,the expres-sion of CDKN2B-AS1,ki67,and VEGF decreased,and the expression of miR-411-3p increased(allP<0.05).Conclusion Inhibition of CDKN2B-AS1 expression can target miR-411-3p levels,thereby inhibiting breast cancer cell proliferation,migration,invasion and tumor growth.