Cord Blood Banking and Transplantation in China: A Ten Years Experience of a Single Public Bank |
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Authors: | Jinhui Liu Ji He Shu Chen Fei Qin Fang Wang Gang Xu Faming Zhu Hangjun Lv Lixing Yan |
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Institution: | a Blood Center of Zhejiang Province, Zhejiang, People''s Republic of China;b Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, People''s Republic of China |
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Abstract: | BackgroundUmbilical cord blood (UCB) has successfully used for transplantation to treat hematologic malignancies and genetic diseases. Herein, we describe the experience generated in a single public UCB bank at Zhejiang Province in China.MethodsGood manufacturing practice and standard operating procedures were used to address donor selection as well as UCB collection, processing, and cryopreservation. Total nucleated cells (TNCs), cellular viability, CD34+ cells, and colony-forming units were determined, and infectious diseases screening test, sterility test, and HLA typing for UCB units were done.ResultsOnly 18.51% of all collected UCB units met storage criteria, and 7,056 UCB units were cryopreserved in 10 years. The volume of UCB units was 95.0 ± 22.0 ml. The number of TNCs before and after processing was 13.32 ± 3.63 × 108 and 10.63 ± 2.80 × 108, respectively, and the recovery rate was 80.71 ± 11.26%. 0.4344 ± 0.1874% of the TNCs were CD34+ cells. The CFU-GM was 32.1 ± 28.0 colonies per 1 × 105 nucleated cells. Based mainly on HLA and nucleated cell content, 26 UCB units were released for transplantation.ConclusionsA public UCB bank was successfully established in China; collection and processing of UCB units should be optimized in order to gain maximum volume and cell count. |
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Keywords: | Umbilical cord blood Cryopreservation Transplantation |
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