Integrin介导的ERK信号传导通路对滋养层细胞侵袭行为的影响

目的：探讨Integrin介导的ERK信号传导通路在滋养层细胞侵袭过程中的作用.方法：利用人绒毛外细胞滋养层细胞体外侵袭模型,将不同浓度的integrin α1和integrin β1混合抗体分别加入绒毛外细胞滋养层细胞（EVCT）培养液中,培养4 h,观察integrin α1和integrin β1混合抗体对EVCT的生长、体外侵袭、FAK和ERK1/2磷酸化的影响.结果：integrin α1和integrin β1混合抗体呈浓度依赖方式抑制EVCT中FAK磷酸化程度,同样呈浓度依赖方式抑制磷酸化的ERK1/2表达,P〈0.05.结论：细胞外基质（ECM）成分能通过细胞表面的相应受体integrin α1β1,激活滋养层细胞中重要的蛋白激酶FAK和ERK.ERK信号传导通路对滋养层细胞的侵袭行为起到重要的调节作用.

Effects of Integrin mediated ERK signaling pathway on invasiveness of trophocytes

Objective：To study the effect of Integrin-mediated ERK signaling pathway on the process of trophocyte invasion. Methods：Extravillous eytotrophoblasts （EVCT） invasion model in vitro was established, and then we put different concentrations of integrin α1 and integrin β1 antibodies into the EVCT medium for 4 h, and observed the effect of integrin α1 and integrin β1 mixed antibodies on growth, in vitro invasion of EVCT, FAK and ERK1 phosphorylation. Resuls：The phosphorylation of FAK in EVCT was dose-dependently inhibited by integrin α1 and integrin β1 combined antibody, and its antibody also dose-dependently inhibited expression of phosphorylated ERK1/2. Conclusions： Extracellular matrix （ECM） may activate FAK and ERK in trophocytes by correspondent integrin α1β1 on cell surface. The ERK signal transduction pathway regulates the invasion of EVCTs.