HLA-G和Fas配体在肾透明细胞癌中的表达及意义

目的：探讨两种免疫抑制分子人类白细胞表面抗原HLA—G和Fas配体（FasL）与经典型肾透明细胞癌（ccRCC）分级和分期的相关性。方法：采用免疫组织化学方法对60例ccRCC标本石蜡切片进行检测，数据由SPSS软件进行统计分析。结果：肿瘤旁正常肾组织中无HLA—G表达；肾癌组织中，40例（66．7％）表达HLA—G，36例（60．0％）表达FasL。31例（51．7％）两者均表达，15例（25．0％）两者均不表达。在同一标本中，肿瘤侵犯脉管或淋巴结后，其HLA-G或FasL表达较原位肿瘤明显增强。统计分析显示HLA—G表达的阳性率和表达强度与肿瘤的分期、分级均呈正相关（P〈0．01）。FasL表达的阳性率与肿瘤分级呈正相关（P〈0．05），而表达强度与肿瘤分期呈正相关（P〈0．01）。结论：ccRCC中HLAG和FasL的表达与肿瘤的分期、分级均呈正相关，与淋巴结转移有一定关系；晚期ccRCC高表达免疫抑制分子的机制需进一步研究。

HLA-G and Fas ligand expression in clear cell renal cell carcinoma

Objective:To investigate the expression of two kind of immunosuppressive molecules, nonclassical human leucocyte antigen class HLA-G and Fas ligand (FasL), in the conventional (clear cell) renal cell carcinoma (ccRCC).Methods:60 cases of ccRCC's formalin-fixed, parafflin embeded surgical specimens were examined by immunohistochemistry. The data were statistically analysed by SPSS software.Results:There was no expression of HLA-G in the normal renal tissue. HLA-G was detected in 40 ccRCC cases ((66.7)%),FasL was detected in 36 cases ((60.0)%). Both the HLA-G and FasL were positive in 31 cases ((51.7)%) and were negative in 15 cases ((25.0)%).Both the HLA-G and FasL expression level were increased after the ccRCC cells invaded the vessel or lymphatic node. The incidence and level of HLA-G were correlated with tumor grade or stage(P<(0.01)). The incidence of FasL expression were correlated with the tumor grade (P<(0.05))but the level of FasL expression were correlated with the tumor stage (P<(0.01)).Conclusions:The expression of HLA-G and FasL were correlated with the tumor stage, grade and metastasis of ccRCC. The mechanisms of high expression of immunosuppressive molecules in the advanced stage ccRCCs were required further investigation.