蒙药验方溃疡散对实验性大鼠胃溃疡的作用

目的:观察蒙药验方溃疡散对大鼠应激性胃溃疡、乙醇导致的胃溃疡及幽门结扎术的胃溃疡等实验性胃溃疡的影响。方法:将150只健康Wistar大鼠分为3个实验组,每个实验组分别分为模型组、溃疡散高、中、低3个剂量组(2.26,1.13,0.56 g.kg-1)和雷尼替丁组(0.15 g.kg-1)或胶体果胶铋组(0.25 g.kg-1)等5个组,每组10只。各给药组每天灌胃1次,连续灌胃7 d,末次给药后各组动物以相应方法致溃疡模型。各组大鼠处死后通过肉眼观察溃疡点状;采用图像分析软件ipWin计算溃疡面积;测定胃液中总酸度和胃蛋白酶活性,观察溃疡散对各型溃疡的影响。结果:溃疡散对应激性胃溃疡面积具有明显的抑制作用(P<0.05或P<0.01);溃疡散对乙醇导致的胃溃疡溃疡点数和溃疡面积均有明显的抑制作用(P<0.05或P<0.01);溃疡散对幽门结扎致胃溃疡大鼠胃液中胃酸总浓度和胃蛋白酶的活性具有一定的抑制作用(P<0.05)。结论:溃疡散对大鼠实验性胃溃疡具有预防作用和胃黏膜保护作用。

Study on the Effect of Mongolian Medicine PRAUP on Experimental Rat Gastric Ulcer

Objective: To observe the effect of Mongolian medicine proved recipe anti-ulcer powder(PRAUP) on three kinds of experimental rats' gastric ulcers, including stress gastric ulcer, alcohol gastric ulcer and pylori-ligation gastric ulcer. Method: Healthy Wistar rats were randomly divided into 3 groups.Each group included 5 small groups as model group, 3 different doses of PRAUP groups(2.26, 1.13, 0.56 g·kg^-1), and Ranitidine group(0.15 g·kg^-1) or Colloidal bismuth pectin group(0.25 g·kg^-1).Each small group consisted of 10 animals.Every medication group was continuously administrated once a day for 7 d.The model group was given same volume of water once a day for 7 d.After the final administration, animals were made the ulcer models.After killed, appearances and areas of rats' ulcers were totally visual studied and taken pictured by ipWin software, gastric total acidities and pepsin activities of the all were measured to research effect of the medicine on the each ulcer. Result: Compared with model group, the PRAUP could reduce areas of stress gastric ulcer of rats evidently (P<0.05 or P<0.01), control amount points and areas of alcohol gastric ulcer of rats markedly (P<0.05 or P<0.01), and could restrain gastric total acidities and pepsin activities of pylori-ligation gastric ulcer of rats (P<0.05). Conclusion: The PRAUP has the obvious preventive effect on experimental rats' gastric ulcer and protective effect on gastric mucosa of rats.