乙肝清对小鼠急性肝损伤的保护作用

目的:研究乙肝清对小鼠急性肝损伤的保护作用。方法:健康小鼠随机分为正常对照组、模型组、联苯双酯组(200 mg.kg-1)、乙肝清高、中、低剂量组生药(30,15,7.5 g.kg-1),每组10只,分别灌胃给药,每天1次,连续10 d。分别采用四氯化碳(CCl4)和D-氨基半乳糖(D-GalN)致小鼠急性肝损伤模型,检测各组动物血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的活性,观察肝组织的病理改变。结果:在CCl4肝损伤实验中,乙肝清中、高剂量能显著降低血清ALT水平(P<0.05或P<0.01),乙肝清高剂量还可降低血清AST水平(P<0.05),明显地改善肝脏的病理损伤;在D-GalN肝损伤实验中,乙肝清高剂量能显著降低血清ALT,AST水平(P<0.05),显著改善肝脏病理损伤。结论:乙肝清对小鼠急性肝损伤有明显的保护作用。

Protective Effects of Yiganqing on Acute Hepatic Injury in Mice

Objective: To study the protective effects of Yiganqing on acute hepatic injury in mice. Method: The healthy mice were divided into six groups at random, ten in each group: normal control group, model group, Bifendate group, Yiganqing high, middle and low dose group(30, 15, 7.5 g·kg^-1 of crude drug, respectively).The mice in treatment groups were intragastric administration respectively, measwhile, the mice in normal control and model groups were treated with the same dose of distilled water, once a day for ten days.The models of liver injury induced by Carbon tetrachloride and D-galactosamine in mice were used.The activities of serum alanine aminotransferase(ALT) and aspartate aminotransferase (AST) were determined, and the pathological change of liver tissue was observed. Result: In the mouse models induced by Carbon tetrachloride, the level of ALT in serum of rats in the middle dose group and the high dose group of Yiganqing was reduced significantly (P<0.05 or P<0.01).The level of AST in serum of rats in the high dose group of Yiganqing was reduced significantly (P<0.05).And the liver pathological injury was alleviated obviously.In the mouse models induced by D-galactosamine, the levels of ALT and AST in serum(P<0.05) could be reduced significantly in the high dose group of Yiganqing and protect the liver from pathological injury. Conclusion: Yiganqing has protective effects on acute hepatic injury in mice.