金莲花总黄酮抗菌作用的实验研究

目的:观察金莲花总黄酮(FTLR)体内外的抗菌活性。方法:体外抗菌实验,采用营养肉汤对金黄色葡萄球菌等14个菌株进行试管连续稀释法和营养琼脂扩散法测定FTLR的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。体内实验采用金黄色葡萄球菌26002-6标准株感染昆明种小鼠作为模型,模型小鼠被随机分为FTLR 440,220,110 mg.kg-1剂量组,金莲花片2 400 mg.kg-1对照组(金莲花片2 400 mg.kg-1与FTLR 440 mg.kg-1的生药剂量相等)及模型对照组,以感染后24,48 h小鼠的死亡率为指标,观察对金黄色葡萄球菌的体内抗菌活性。结果:在体外抑菌实验中,FTLR对13个细菌标准菌株和10个临床株均有抑制作用,其MIC和MBC均低于双黄连口服液;在体内抑菌实验中,FTLR各剂量组金黄色葡萄球菌感染小鼠48 h死亡率均明显低于模型对照组,440 mg.kg-1组死亡率低于金莲花片2 400 mg.kg-1组。结论:对多种细菌有明显的抑制作用。相等生药量的总黄酮对金黄色葡萄球菌感染小鼠的保护作用强于金莲花片。

Study on Antibacterial Activity of Flavonoids from Trollius ledebouri

Objective: To study the antibacterial activity of the flavonoids from Trollius ledebouri Reichb (FTLR) were observed in vivo and in vitro. Method: By methods of tube continuous dilution and agar plate diffusions to 14 strains of bacteria, for example Staphylococcus aureus, minimal bacteriostatic concentration (MIC) and minimal bactericidal concentration (MBC) of FTLR were determined in vitro. To evaluate antibacterial activity of FTLR in vivo, the model of mice infected by the type strain of S. aureus 26002-6 was preparated and they were randomly divided into groups of 440, 220, 110 mg·kg^-1 of FTLR, 2 400 mg·kg^-1 Jinlianhua tablet (2 400 mg·kg^-1 of Jinlianhua tablet and 440 mg·kg^-1 of FTLR were the same of crude drug dose)and model control group and the mortality of mice in different groups was calculate in 24, 48 h mice after infection. Result: In bacteriostasis experiment in vitro, FTLR shows obvious bacteriostatic activity on the used strains and its MIC and MBC were lower than those of Shuanghuanglian oral liquid. In bacterostasis activity in vivo, the mortality of mice in all groups of FTLR was lower than in modle control group in 24, 48 h mice after infection. Conclusion: FTLR has obvious inhibition effect on several kinds of bacteria in vitro and protective effect on infected mice induced by S.aureu 26002-6.