参莲提取物对大鼠腹腔肥大细胞释放活性物质的干预作用

目的:探讨参莲提取物对大鼠腹腔肥大细胞释放活性物质的干预作用。方法:提取原代大鼠腹腔肥大细胞,纯化后以5×106个/mL浓度接种于培养板中,加参莲提取物预处理2 h后,用C48/80刺激肥大细胞脱颗粒,ELISA法检测细胞上清液以及细胞裂解液中组胺(histamine),类胰蛋白酶(tryptase),以及5-羟色胺(5-hydroxytryptamine,5-HT)的含量,计算活性物质释放率。结果:模型组较对照组组胺释放率由32.36%升至61.71%(P<0.01),参莲提取物各剂量(100,50,25,12.5 mg.L-1)作用后,组胺释放率分别降至16.24%,26.25%,27.81%,31.50%(P<0.01);模型组较对照组类胰蛋白酶释放率由13.19%升至21.72%(P<0.01),参莲提取物各剂量(100,50,25,12.5 mg.L-1)作用后,类胰蛋白酶释放率分别降至8.60%,15.78%,8.80%,12.15%(100,25,12.5 mg.L-1组P<0.01,50 mg.L-1组P<0.05);模型组较对照组5-HT释放率没有明显变化,但参莲提取物100,50,25 mg.L-1组显著增加5-HT释放率(P<0.01,P<0.05)。结论:参莲提取物对大鼠腹腔肥大细胞有一定的稳定作用。

Effects of Shenlian Extracts on Rat Peritoneal Mast Cells Activation

Objective: To investigate the effects of Shenlian (SL) extracts on mast cells activation. Method: Rat peritoneal mast cells (RPMC) were collected and seeded on 96-well plates. Compound 48/80 was added into the cultural supernatants after the cells were pretreated with SL extracts for 2 h. Then we collected the supernatants and detected Histamine, 5-hydroxytryptamine and tryptase content with ELISA kits. Result: The results show Compound 48/80 increased histamine release from 32.36% to 61.71% (P<0.01). SL extracts(100, 50, 25, 12.5 mg·L^-1) reduced RPMC histamine release to 16.24%, 26.25%, 27.81%, 31.50%(P<0.01). Compound 48/80 increased tryptase release from 13.19% to 21.72% (P<0.01), SL extracts(100, 50, 25, 12.5 mg·L^-1) reduced RPMC tryptase release to 8.60%, 15.78%, 8.80%, 12.15% (100, 25, 12.5 mg·L^-1, P<0.01, 50 mg·L^-1, P<0.05). Compound 48/80 had no obvious effect on RPMC 5-HT release. But 100, 50, 25 mg·L^-1 of SL extracts can increase 5-HT release (100, 50 mg·L^-1, P<0.01; 25 mg·L^-1, P<0.05). Conclusion: We concluded SL extracts can stabilize mast cells.