| ICP-OES法与EDTA直接滴定法测定含石膏方剂中钙离子含量研究 |
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| 引用本文： | 李晓晶,马强,盛振华,葛尔宁,苏燕.ICP-OES法与EDTA直接滴定法测定含石膏方剂中钙离子含量研究[J].中国实验方剂学杂志,2013,19(6):69-74. |
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| 作者姓名： | 李晓晶 马强 盛振华 葛尔宁 苏燕 |
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| 作者单位： | 内蒙古科技大学包头医学院 基础学院,内蒙古 包头 014060;中国医学科学院 基础医学研究所,北京协和医学院 基础学院生物医学工程学系,北京 100005;浙江中医药大学 分析测试中心,杭州 310053;浙江中医药大学 分析测试中心,杭州 310053;内蒙古科技大学包头医学院 基础学院,内蒙古 包头 014060 |
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| 基金项目： | 国家自然科学基金项目(81160214);教育部科学技术研究重点项目(210039);内蒙古自然科学基金项目(2011MS1132;200711020913) |
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| 摘 要： | 目的:通过对比EDTA直接滴定法和ICP-OES法测定钙标准液、单煎石膏药液、白虎汤、麻杏石甘汤和竹叶石膏汤同一样品中Ca2+溶出量差异,探讨复方汤剂中有机基底等因素对EDTA直接滴定法测定复方药液中Ca2+溶出量的影响程度并建立一种可信的测定方剂中Ca2+溶出量的方法。方法:通过对比EDTA直接滴定法和微波消解-ICP-OES法测定钙标准液、单煎石膏药液、白虎汤、麻杏石甘汤和竹叶石膏汤同一样品中Ca2+溶出量。结果:EDTA直接滴定法与ICP-OES法测定钙标准液结果间无显著差异,两方法测定结果相同。两方法测定单煎石膏、白虎汤、麻杏石甘汤和竹叶石膏汤样品中Ca2+溶出量结果间差异显著(P<0.01),EDTA直接滴定法测定结果较ICP-OES法偏大。结论:EDTA直接滴定法测定样品中Ca2+溶出量受样品基底复杂程度影响,影响因素对EDTA直接滴定法测定影响均匀且呈线性关系。EDTA直接滴定法测定复方中Ca2+溶出量偏大,较不适合用于测定中药复方中Ca2+溶出量。
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| 关 键 词： | EDTA直接滴定法 ICP-OES法 石膏 含石膏方剂 方法学比较 |
| 收稿时间： | 2012/7/20 0:00:00 |
Comparison of ICP-OES and EDTA Titration Methods of the Results of Calcium Content in Gypsum Formulae |
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| LI Xiao-jing,MA Qiang,SHENG Zhen-hu,GE Er-ning and SU Yan.Comparison of ICP-OES and EDTA Titration Methods of the Results of Calcium Content in Gypsum Formulae[J].China Journal of Experimental Traditional Medical Formulae,2013,19(6):69-74. |
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| Authors: | LI Xiao-jing MA Qiang SHENG Zhen-hu GE Er-ning and SU Yan |
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| Institution: | Basic Medical College, Baotou Medical College, Inner Mongolia University of Science & Technology, Baotou 014060, China;Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China;Analytical Testing Center, Zhejiang Chinese Medical University, Hangzhou 310053, China;Analytical Testing Center, Zhejiang Chinese Medical University, Hangzhou 310053, China;Basic Medical College, Baotou Medical College, Inner Mongolia University of Science & Technology, Baotou 014060, China |
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| Abstract: | Objective: The Ca2+ content in calcium standard solution, the gypsum decoction, Baihu decoction, Maxing Shigan decoction and Zhuye Shigao decoction was determined by comparing the difference of EDTA titration method and ICP-OES method to discuss the effect of organic matric and other factors of decoction with the degree of Ca2+ content on using EDTA titration and to establish a credible method of Ca2+leaching quantity of the decoction. Method: The EDTA titration method and the ICP-OES method results were compared to determine Ca2+ content of the same sample in calcium standard solution, gypsum decoction, Baihu decoction, Maxing Shigan decoction and Zhuye Shigao decoction. Result: The results of the calcium standard solution between EDTA titration method and ICP-OES determination were no significant(P>0.05), two methods were the same results.The results of Ca2+ content were significantly different with the determination of two methods for gypsum, Baihu decoction, Maxing Shigan decoction and Zhuye Shigao decoction(P<0.01).The value of results by EDTA direct titration method was higher than that by ICP-OES method. Conclusion: Ca2+content determination by using EDTA titration is affected with the complex degree of sample's organic matrices, influence factors on the determination of EDTA titration methods are homogeneous and linear relationship.The Ca2+ content determination result value of decoction is high by EDTA direct titration method, and it is less suitable for the determination of Ca2+ content of the traditional medical formulae. |
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| Keywords: | EDTA direct titration method ICP-OES method gypsum containing gypsum formula methodology comparison |
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