TLR9抑制剂对胃癌细胞MGC803分泌IL-6的作用

目的:探讨免疫系统蛋白TLR9在胃癌细胞MGC803中的表达及其抑制剂氯喹(chloroquine,CQ)作用胃癌细胞MGC803不同时间段对其分泌白细胞介素-6(IL-6)的影响。方法:应用免疫荧光染色的方法检测胃癌MGC803细胞中TLR9的表达;运用CCK-8法检测CQ作用细胞24,48,72 h后,观察药物对细胞活性的影响;ELISA方法检测不同浓度CQ作用MGC803细胞不同时间点后,细胞上清液中IL-6的活性水平。结果:免疫荧光染色方法显示胃癌MGC803细胞上有TLR9的表达;CCK-8法检测不同浓度的抑制剂作用细胞3个时间段后对细胞的生长均具抑制作用,差异有显著性(P<0.05);ELISA结果表明,200 mg.L-1CQ作用细胞24,48,72 h后均可抑制IL-6的表达,而50,100 mg.L-1的CQ仅在作用细胞72 h后抑制IL-6的表达,且差异具显著性(P<0.05)。结论:胃癌细胞系MGC803表达有TLR9;TLR9抑制剂CQ可抑制IL-6的表达。

Research of TLR9 Inhibitor Influenced Release of IL-6 on Gastric Cancer Cell MGC803

Objective: To investigate the expression of TLR9 in gastric cancer cell line MGC803 and the influence of TLR9 inhibitor chloroquine (chloroquine, CQ) treated the MGC803 cell in different period on the secretion of interleukin-6(IL-6). Method: To Measure TLR9 expression in MGC803 gastric cancer cell line by immunofluorescence staining.Observed the effect of CQ in MGC803 cell at 24, 48, 72 h by CCK-8 assay.The bioactivity of IL-6 in the MGC803 cell suspension treated with different concentration CQ in three period was detected by ELISA. Result: MGC803 cells expressed TLR9.Different concentration of CQ could inhibit the growth of MGC803 cells (P<0.05) at different time point.200 mg·L^-1 CQ decreased the bioactivity of IL-6 in 24, 48, 72 h, but 50, 100 mg·L^-1 CQ suppressed IL-6 expression only after 72 h(P<0.05). Conclusion: MGC803 cells expressed TLR9 and TLR9 inhibitor CQ could inhibit IL-6 bioactivity.