兔脊柱VX2移植瘤模型的建立及生物学特性观察

目的探讨兔脊柱VX2移植瘤模型的建立方法及其生物学特性。方法新西兰大白兔25只，分为两组。实验组（20只）采用VX2瘤细胞悬液经横突椎弓根注射法建立兔脊柱VX2移植瘤动物模型，对照组（5只）以生理盐水注射代替VX2瘤细胞悬液进行同样操作。接种后每天观察下肢神经功能变化，接种术后第2周起每周行X线、CT影像学检查肿瘤生长情况。动物出现下肢瘫痪、死亡或接种后6周为观察终点，取出接种椎作组织学检查。结果对照组5只兔观察期内均未出现神经功能障碍，检查未见异常。实验组中1只兔因钻孔破人椎管，退出实验。19只兔成功接种，1只于接种术后9天感染死亡，1只经病理检查证实观察截止时仍无肿瘤生长，其余17只成瘤（89．5％）。观察期内9只出现完全瘫痪，5只不全瘫，其余3只未出现神经功能障碍。结论经横突-椎弓根种植法建立兔脊柱VX2移植瘤模型具有成瘤率高、可重复性好的特点。该模型能较好地模拟脊柱转移癌发展、压迫脊髓导致瘫痪的病理过程，有助于脊拄转移癌各种治疗模式的研究和探索。

VX2 transplanted carcinoma in rabbit spine:establishing model and observing biologic feature

Objective To establish spinal transplanted tumor model of VX2 carcinoma in rabbit and to observe its biological feature.Methods Twenty-five New Zealand white rabbits were divided into 2 groups.The 20 rabbits of experimental group were injected with suspension containing VX2 carcinoma cells into the vertebral body through a trans-transverse process and pedicle approach,while the 5 rabbits of control group underwent the same injection except that suspension was replaced by normal sodium.Motor function of lowe...