Evaluation of LAK-mediated tumor cell killing in a plasma clot clonogenic assay |
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Authors: | A Dinota P L Tazzari A Bontadini D Raspadori C Tassi L Zucchini M Pizzuti F Ricciuti M Gobbi |
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Institution: | Divisione di Ematologia, Ospedale S. Carlo, Bologna, Italy. |
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Abstract: | An assay based on the inhibition of the cloning capacity in a plasma clot semisolid medium assay has been used to test the sensitivity of the Raji cell line to lymphokine-activated killer (LAK) cells. This method overcomes some limitations intrinsic to the widely employed 51Cr release assay and always shows a higher degree of sensitivity. No inhibition of colony growth was found when the effector cells were plated without prior pre-incubation with interleukin 2 or with the addition of the medium derived from the LAK cells. Though more time-consuming than the classic 51Cr release assay, this technique does not require radioactive material. This test may be suitable for a more precise evaluation of LAK activity and for the study of the mechanisms involved in cell killing. |
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