| Significance and Expression of Serum and Glucocorticoid-inducible Kinase in Kidney of Mice with Diabetic Nephropathy |
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| 作者姓名： | 王全胜 张晓丽 王玉梅 邓安国 朱忠华 冯玉锡 |
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| 作者单位： | [1]DepartmentofChineseTraditionalMedicine,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022,China [2]DepartmentofNephrology,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022,China |
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| 基金项目： | This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30270618). |
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| 摘 要： | To investigate the expression and the role of three isoforms of Serum and Glucocorticoidinducible Kinase (SGK) in experimental diabetic nephropathy (DN), 12 male C57BL/6 mice of 8-weeks-old were divided into two groups. Streptozotocin (STZ)-induced diabetic nephropathy and normal controls were analyzed at the end of the 4th week after the induction of diabetes. Renal hemodynamics and histological studies were performed. The expression of SGK1 mRNA, SGK2 mRNA and SGK3 mRNA of kidney cortex were measured by RT-PCR, and the cortical SGK1 protein was detected with Western blotting. Our results showed that the blood glucose, blood HbA1c, 24h urinary protein, creatinine clearance and the renal index were all increased in DN group. More extracellular matrix (ECM) accumulation was observed. The level of cortical SGK1 mRNA and protein were up-regulated in DN group in comparison with control group. SGK2 and SGK3 mRNA were elevated in DN mice. In DN, mRNA level of three SGK isoforms and SGK1 protein were increased significantly. It is concluded that SGKs may contribute to the early renal injury of DN.
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| 关 键 词： | 肾上腺皮质激素 激酶 糖尿病 肾疾病 免疫血清 小鼠 动物实验 |
| 收稿时间： | 20 December 2004 |
Significance and expression of Serum and Glucocorticoid-inducible Kinase in kidney of mice with diabetic nephropathy |
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| Wang Quansheng,Zhang Xiaoli,Wang Yumei,Deng Anguo,Zhu Zhonghua,Feng Yuxi.Significance and Expression of Serum and Glucocorticoid-inducible Kinase in Kidney of Mice with Diabetic Nephropathy[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(2):170-173. |
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| Authors: | Wang Quansheng Zhang Xiaoli Wang Yumei Deng Anguo Zhu Zhonghua Feng Yuxi |
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| Institution: | 1. Department of Chinese Traditional Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
2. Department of Nephrology , Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430022, China |
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| Abstract: | Summary: To investigate the expression and the role of three isoforms of Serum and Glucocorticoid-inducible Kinase (SGK) in experimental diabetic nephropathy (DN), 12 male C57BL/6 mice of 8-weeks-old were divided into two groups. Streptozotocin (STZ)-induced diabetic nephropathy and normal controls were analyzed at the end of the 4th week after the induction of diabetes. Renal hemodynamics and histological studies were performed. The expression of SGK1 mRNA, SGK2 mRNA and SGK3 mRNA of kidney cortex were measured by RT-PCR, and the cortical SGK1 protein was detected with Western blotting. Our results showed that the blood glucose, blood HbA1c, 24-h urinary protein, creatinine clearance and the renal index were all increased in DN group. More extracellular matrix (ECM) accumulation was observed. The level of cortical SGK1 mRNA and protein were up-regulated in DN group in comparison with control group. SGK2 and SGK3 mRNA were elevated in DN mice. In DN, mRNA level of three SGK isoforms and SGK1 protein were increased significantly. It is concluded that SGKs may contribute to the early renal injury of DN. |
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| Keywords: | serum and glucocorticoid-inducible kinase diabetic nephropathy |
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