γδT细胞在铜绿假单胞菌肺炎患者外周血和痰液中的分布变化的探讨

目的：了解铜绿假单胞菌肺炎患者痰、外周血中γδT细胞的变化，探索γδT细胞在铜绿假单胞菌肺炎中的作用。方法：铜绿假单胞菌肺炎患者8例（A组），正常对照者8例（B组），采集受试者的外周血和下呼吸道痰液，运用手工计数方法计数各种细胞数量并计算各种细胞占总细胞中的比例。运用流式细胞技术检测淋巴细胞占总细胞中的百分比及γδT细胞占淋巴细胞中的百分比。结果：A组外周血和痰液中γδT细胞数量占淋巴细胞的百分比均明显高于B组（P〈0.01）；A组外周血γδT细胞占淋巴细胞中的百分比低于痰液中γδT细胞占淋巴细胞中的百分比（P〈0.01），而B组外周血和痰液中γδT细胞占淋巴细胞中的百分比差异无统计学意义（P〉0.05）。结论：γδT细胞可能通过重新分布在铜绿假单胞菌肺炎发病过程中发挥作用。

GAMMADELTA T CELLS AND OTHER IMMUNE CELLS IN PERIPHERAL BLOOD AND SPUTUM OF PATIENTS WITH PSEUDOMONAS AERUGINOSA PNEUMONIA AND A STU- DY ON THEIR CHANGES OF DISTRIBUTION

Objective：To understand bacteria pseudomonas aeruginosa pneumonia in peripheral blood of pa- tients with sputum, gammadelta T cells, neutrophils and lymphocytes, explore these cells in the role of the bacterium pseudomonas aeruginosa pneumonia disease. Methods： There were 8 patients with pseudo- monas aeruginosa pneumonia （group A） and 8 healthy persons （group B）. Their peripheral blood and spu- tum of under air way were collected. The cell amount and the percentage of total cells were calculated, and the lymphocyte and gammadelta T cells were detected by flow cytometry. Results： For group A, the per- centage of gammadelta T cells in lymphocyte in peripheral blood was lower than that in sputum （ P 0. 05）. But for group B, there was no difference in the distribution of gammadelta T cells in peripheral blood and in sputum （ P ~0.05）. Conclusion： Gammadelta T cells may play an important role in pseudo- monas aeruginosa pneumonia through redistribution in this process.