高效液相色谱法同时测定扁咽口服液中黄芩苷和肉桂酸的含量

目的建立高效液相色谱法同时测定扁咽口服液中黄芩苷和肉桂酸的含量。方法色谱柱：Agilent Eclipse XDB—C18（4．6mm×150mm，5μm）；流动相：乙腈-2％冰醋酸溶液（23：77）；流速：1．0ml／min；检测波长：280nm；柱温：室温；进样量：10μl。结果黄芩苷和肉桂酸分别在0．0851～3．4048g（r=0．9999）和0．0203～0．8112g（r=0．9999）范围内与其峰面积线性关系良好（r=0．9999），平均回收率（n=6）为101．68％和101．46％，RSD为285％和2．07％。结论本方法快速、简便、准确，能更全面控制和评价扁咽口服液的质量。

Determination of baicalin and cinnamic acid in Bianyan oral solution by HPLC

Objective To establish an HPLC method for determination of baicalin and cinnamie acid in Bianyan oral solution simultaneously. Methods The Agilent Eclipse XDB-C18 （4.6 mm × 150 mm, 5 μm）was used, the mobile phase consisted of ACN- 2% acetic acid （23 ： 77） , the flow rate was 1.0 ml/min, the detection wavelength was at 280 nm, the column temperature was at room temperature, and the inject volume was 10 μl. Results A good linear correlation was observed within the range of 0. 085 1 3. 404 8 g for baicalin （ r = 0. 999 9） and 0. 020 3 - 0.811 2 g for cinnamic acid （ r = 0. 999 9）. The average recovery （ n = 6） was 101.68% with RSD 2.85% for baicalin and 101.46% with RSD 2.07% for cinnamic acid. Conclusion The method was rapid,simple and accurate,which could be used for the overall quality control of Bianyan oral solution.