核酸疫苗Sj31BIN联合IL-12免疫抗日本血吸虫攻击感染的实验研究

目的 :在证明日本血吸虫重组蛋白酶B核酸疫苗Sj31BIN具有抗生殖免疫作用的基础上 ,联合使用IL 12 ,观察IL 12是否具有佐剂效果。方法 :分别用Sj31BIN +IL 12和IL 12免疫Balb/C小鼠。攻击感染后 6周计数成虫负荷和组织内虫卵数及肝脏表面虫卵结节数。结果 :Sj31BIN +IL 12免疫小鼠可降低成虫发育率。肝组织减卵率为5 9 74% ;肠组织减卵率为 5 9 6 0 % ;肝脏表面虫卵结节减少率为 71 30 % ;单独使用IL 12有一定的免疫保护作用。结论 :Sj31BIN +IL 12能诱导小鼠产生较强的抗生殖免疫作用。

erimental study on immunization of mice with nucleic acid vaccine Sj31BIN combining IL-12 against Schistosoma japonicum

Based on the anti fecundity effect of Schistosoma japonicum nucleic acid vaccine Sj31BIN, we combined Sj31BIN with IL 12 in vaccination of mice to explore the role of IL 12 as an adjuvant. The result showed that immunization of the mice with Sj31BIN+IL 12 led to a significant decrease in adult worm recovery, a liver egg count reduction of 59.74%, an intestine egg count reduction of 59.60% and a liver surface egg granuloma reduction of 71.3%. In addition, vaccination of the mice with IL 12 alone also led to some but not significant decrease in adult worm recovery and the egg counts. It is concluded that Sj31BIN plus IL 12 induces significant anti fecundity immunity.