TRAIL诱导神经母细胞瘤细胞凋亡中 Caspase 8活性的变化

目的：探讨肿瘤坏死因子相关凋亡诱导配体（TRAIL）诱导神经母细胞瘤细胞株CHP212细胞凋亡过程中Caspase 8活性的变化.方法：应用流式细胞仪检测TRAIL、Caspase 8抑制剂（zIETD-FMK）＋TRAIL对CHP212细胞的诱导凋亡作用 应用比色法测定Caspase 8相对活性 应用透射电镜对凋亡细胞进行形态学观察.结果：CHP212细胞对TRAIL的诱导凋亡作用敏感，存在剂量依赖性.随TRAIL作用时间的延长，Caspase 8活性逐步升高，于作用16h达高峰 不同浓度TRAIL处理组Caspase 8相对活性随浓度增加而递增.zIETD-FMK能阻断Caspase 8的活化而抑制TRAIL对CHP212细胞的诱导凋亡作用.透射电镜可见到典型的细胞凋亡特征.结论：TRAIL通过Caspase 8信号传导通路诱导CHP212细胞凋亡并伴随Caspase 8活性增高.

Change of Caspase 8 Activity in TRAIL-induced Apoptosis of Neuroblastoma Cells

Objective:To study the change of Caspase 8 activity in TRAIL-induced apoptosis of CHP212 neuroblastoma cells.Methods:The effects of TRAIL and Caspase 8 inhibitor zIETD-FMK+ TRAIL on the apoptosis of CHP212 cells were detected using flow cytometry.Relative Caspase 8 activ- ity was measured by colorimetric assay.Apoptotic cells were visually detected using a transmission electron microscope(TEM).Results:CHP212 cells were sensitive to TRAIL and this sensitivity was dose-dependent.The relative Caspase 8 activity in CHP212 cells increased gradually with prolonged exposure to TRAIL and with increased TRAIL concentration.The zIETD-FMK inhibitor could block the activation of Caspase 8 and diminish the TRAIL-induced apoptosis of CHP212 cells.Typical fea- tures of apoptosis were seen under TEM.Conclusion:TRAIL could induce the apoptosis of CHP212 cells through a Caspase 8 signal transduction pathway,with an increase in Caspase 8 activity.