血管内皮生长因子及内皮抑素在分化型甲状腺癌手术前后及复发时的表达

目的 探讨血管内皮生长因子(VEGF)及内皮抑素在分化型甲状腺癌手术前后及复发时的表达.方法 分化型甲状腺癌术后肺转移患者7例(肺转移组),局部复发患者21例(局部复发组),选择甲状腺组织结构及功能正常者30例为对照组,分别采用酶联免疫吸附法检测血清VEGF及内皮抑素,化学发光法检测血清甲状腺球蛋白的浓度,应用SPSS 13.0统计学软件包分析,计量资料数据比较应用t检验,VEGF、内皮抑素与甲状腺球蛋白浓度的相关性采用Pearson等级相关分析.结果 肺转移组手术前VEGF、内皮抑素分别为(412.6±57.3)、(34.3±7.6) ng/L,术后3周分别为(210.3 ±30.4)、(25.2 ±6.2)ng/L,复发时分别为(619.5±126.4)、(38.6±8.7) ng/L；局部复发组手术前VEGF、内皮抑素分别为(399.4 ±56.3)、(35.2 ±6.8)ng/L,术后3周分别为(209.3 ±36.7)、(25.7 ±4.7) ng/L,复发时分别为(406.5±59.2)、(31.2±7.6) ng/L,组内比较术后3周显著低于手术前和复发时(P均＜0.05),组间比较复发时差异均有统计学意义(P均＜0.05).甲状腺癌术后复发患者血清甲状腺球蛋白与VEGF及内皮抑素表达成正相关(r值分别为0.752、0.349,P均＜0.01).结论 VEGF、内皮抑素在分化型甲状腺癌术前及术后复发时高表达,可作为评估分化型甲状腺癌生物学行为的一项重要指标.

Study on the expression of vascular endothelial growth factor and endostatin in the serum of patients with differentiated thyroid cancer at the time of before and after operation and postoperative recurrence

Objective To evaluate the expression of vascular endothelial growth factor （VEGF） and endostatin （ES） in the serum of patients with differentiated thyroid cancer at the time of before and after operation and postoperative recurrence. Methods The serum samples were obtained from 7 patients with postoperative lung metastasis and 21 patients with local recurrence after operation. The serum samples from 30 normal subjects were obtained as control. The levels of serum VEGF and serum endostatin were analyzed by enzyme-linked immunosorbent assay （ELISA）. The levels of serum thyroglobulin were analyzed by Chemiluminescence method. Statistical analysis was performed with t-test using SPSS 13.0 software. Pearson correlation analysis was performed for the correlation between serum VEGF and serum ES and thyroglobulin. Results The levels of serum VEGF and serum ES in patients with lung metastasis 3 weeks after operation were significantly lower than those before operation and at the time of recurrence [ VEGF： （210. 3 ＋ 30.4） ng/L vs （412.6 ＋57. 3） ng/L and（619. 5 ± 126.4） ng/L,P 〈0. 05 ;ES： （25.2 26. 2） ng/L vs （34. 3 ＋7. 6） ng/L and （38. 6 ＋ 8.7） ng/L, P 〈 0.05 ]. And the levels of serum VEGF and serum ES in patients with local regional recurrence 3 weeks after operation were significantly lower than those before operation and at the time of recurrence [ VEGF ： （209. 3 ± 36.7 ） ng/L vs （ 399.4 ± 56. 3 ） ng/L and （406. 5 ± 59. 2 ） ng/L, P 〈 0. 05 ; ES ： （25.7 ± 4.7 ） ng/L vs （ 35.2 ± 6. 8 ） ng/L and （ 31.2 ±7.6） ng/L, P 〈 0.05 ]. The expression of serum VEGF and serum ES in patients with pulmonary metastasis were significantly higher than those in patients with local regional postoperative recurrence （ P 〈 0. 05 ）. There was a linear positive correlation between serum VEGF, serum ES and thyroglobulin levels in the patient with recurrent differentiated thyroid cancer（ r = O. 752,0. 349, P 〈0. 01 ）. Conclusion The serum VEGF and serum ES level were significantly elevated in patients with differentiated thyroid cancer before operation and recurrence. The serum VEGF and serum ES were important indicators to reveal the biological behaviors of differentiated thyroid cancer.