| 人重组骨形成蛋白-2诱导NIH3T3细胞向成骨细胞表型转化 |
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| 引用本文： | 王军琳,刘源,金岩,王新文,李媛. 人重组骨形成蛋白-2诱导NIH3T3细胞向成骨细胞表型转化[J]. 华西口腔医学杂志, 2004, 22(5): 420-422 |
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| 作者姓名： | 王军琳 刘源 金岩 王新文 李媛 |
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| 作者单位： | 第四军医大学口腔医学院组织病理学教研室，陕西西安710032 |
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| 摘 要： | 目的探讨人重组骨形成蛋白一2 (rhBMP-2)对NU13T3成纤维细胞生物学行为的影响。方法培养 NIH3T3细胞，加人外源性rhBMP-2，用生长曲线、唾哇蓝(MIT)比色、流式细胞仪(FCM)分析、BrdU检测观察细胞的增殖和DNA合成，并检测碱性磷酸酶(ALP)活性和骨钙素(OC)含量观察细胞是否有成骨趋向。结果加药组细胞的增殖活性下降、DNA合成变慢，All]活性和OC含量均增高。结论rhBMP-2使NIH3T3细胞增殖活性变弱，表现成骨细胞表型。
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| 关 键 词： | 成纤维细胞 骨形成蛋白 成骨细胞 |
| 文章编号： | 1000-1182(2004)05-0420-03 |
| 收稿时间： | 2004-10-25 |
| 修稿时间： | 2003-12-16 |
Recombinant Human Bone Morphogenetic Protein-2 Transform NIH3T3 Fibroblast to Osteo-like Cells |
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| WANG Jun-lin,LIU Yuan,JIN Yon,WANG Xin-wen,U Yuan.. Recombinant Human Bone Morphogenetic Protein-2 Transform NIH3T3 Fibroblast to Osteo-like Cells[J]. West China journal of stomatology, 2004, 22(5): 420-422 |
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| Authors: | WANG Jun-lin LIU Yuan JIN Yon WANG Xin-wen U Yuan. |
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| Affiliation: | Dept.of Oral Histology and Pathology, The Fourth Military Medical Ummsay, Xi'an 710032, China |
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| Abstract: | OBJECTIVE: To examine the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on NIH3T3 cells. METHODS: The NIH3T3 cells were cultured in DMEM with 50 microg/L rhBMP-2. The proliferation ability, alkaline phosphatase (ALP) activity and osteocalcin (OC) quantity of NIH3T3 cells were investigated. RESULTS: The cells showed decreased proliferation ability but enhanced ALP activity and OC quantity. CONCLUSION: 50 microg/L rhBMP-2 can transform NIH3T3 fibroblasts to osteo-like cells. |
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| Keywords: | fibroblast bone morphogenetic proteins osteoblast |
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