| 单克隆抗体鉴别日本血吸虫感染性钉螺的初步研究 |
| |
| 引用本文： | 郑志国,楼涤,张素娥,蒋建敏,汤益,沈炳荣,屠乐鸣,娄文娴. 单克隆抗体鉴别日本血吸虫感染性钉螺的初步研究[J]. 中国血吸虫病防治杂志, 1997, 0(1) |
| |
| 作者姓名： | 郑志国 楼涤 张素娥 蒋建敏 汤益 沈炳荣 屠乐鸣 娄文娴 |
| |
| 作者单位： | 浙江省医学科学院寄生虫病研究所!杭州，310013，浙江省医学科学院寄生虫病研究所!杭州，310013，浙江省医学科学院寄生虫病研究所!杭州，310013，浙江省医学科学院寄生虫病研究所!杭州，310013，浙江省医学科学院寄生虫病研究所!杭州，310013，浙江省医学科学院寄生虫病研究所!杭州，3100 |
| |
| 基金项目： | 卫生部与TDR联合科研管理委员会资助!JRMC |
| |
| 摘 要： | 在筛选McAb与受检钉螺样本的基础上，建立了McAb－ELISA，鉴别钉螺体内日本血吸虫抗原。每只受检钉螺．事先镜检作为对照，感染血吸虫钉螺36只，血吸虫抗原阳性检出率97.22％（35／36）。合并感染其他寄生生钉螺7只，其阳性检出率为100％（7／7）。感染其他寄生虫钉螺8只，无感染钉螺22只，血吸虫抗原阴性符合率均为100％。以上结果表明，McAb－ELISA与镜检法所得的结果是一致的，这为现场检测钉螺感染血吸虫与否将提供一种简便、有效的方法。
|
| 关 键 词： | 日本血吸虫 钉螺 单克隆抗体 ELISA 特异性抗原 |
A PRELIMINARY STUDY ON IDENTIFICATION OF SNAILS INFECTED WITH SCHISTOSOMA JAPONICUM BY MONOCLONAL ANTIBODY |
| |
| Zheng Zhiguo, Lou Di, Zhang Suo,Jiang Jianmin,Tang Yi,Shen Bingrong, Tu Leming,Lou Wenxian. A PRELIMINARY STUDY ON IDENTIFICATION OF SNAILS INFECTED WITH SCHISTOSOMA JAPONICUM BY MONOCLONAL ANTIBODY[J]. Chinese journal of schistosomiasis control, 1997, 0(1) |
| |
| Authors: | Zheng Zhiguo Lou Di Zhang Suo Jiang Jianmin Tang Yi Shen Bingrong Tu Leming Lou Wenxian |
| |
| Abstract: | The McAb-ELISA was established to identify Schistosoma japonicum antigens in snails.The positive rate of schistosoma antigen was 97. 22% in 36 snails infected with schistosome only,and 100% in 7 snails infected with both schistosomes and other parasites. the negative rate of schistosome antigen was 100% in 8 snails infected with other parasites and 22 uninfected snails. Each snail was examined microscopically for the parasite as contrast. The results showed that identification of infected snails with McAb-ELISA was consistent with the microscopic examination. The McAb-ELISA seems to be a simple and effective method for detection of infected snails with schistosome in field. |
| |
| Keywords: | Schistosoma japonica snail monoclonal antibody ELISA specific antigen |
| 本文献已被 CNKI 等数据库收录！ |
