肾细胞癌血管内皮生长因子及微血管密度的免疫组化研究

目的　探讨血管内皮生长因子（VEGF）和微血管生成与肾癌发展的关系。　方法　应用免疫组化技术检测46例肿瘤组织中的VEGF。　结果　46例肿瘤组织中VEGF阳性表达28例 (60.8%)、微血管密度（MVD）为63.64±33.20,均显著高于正常组织。VEGF、MVD与肿瘤的组织类型无关（P＞0.05)，与肿瘤的组织学分级有关（P＜0.05)；VEGF阳性组MVD明显高于阴性组(P＜0.05)；有淋巴结转移组VEGF、MVD均高于无淋巴结转移组(P＜0.05)；VEGF阳性组术后5年复发转移率明显高于阴性组（P＜0.01)。　结论　VEGF与肾癌的血管生成有关，VEGF和MVD可作为反映肾细胞癌生物学行为的指标之一。

Expression and significance of vascular endothelial growth factor and microvessel density in renal cell carcinoma

Objective To elucidate the expression and significance ofvascular endothelial growth factor (VEGF) and the microvessel density (MVD) in renal cell carcinoma. Methods The expression of VEGF and MVD in 46 renal cell carcinoma specimens were detected by immunohistochemistry. Results VEGF expression was positive in 28 of 46 cases of renal cell carcinoma, and MVD 63.64±33.20. No correlation was detected among different pathological types of renal cell carcinoma in expression of VEGF and MVD. There was a high difference in grading of renal cell carcinoma. MVD was higher in VEGF expression group than in VEGF nonexpression group. VEGF expression and MVD was correlated well with metastasis. The 5-year rate of metastasis and recurrence was higher in VEGF expession group than nonexpression group. Conclusions Expression of VEGF and MVD is related to the biological behaviors of renal cell cancinoma. Expression of VEGF is related to angiogenenesis in renal cell carcinoma.