| 新型腺病毒载体的制备及其特性研究 |
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| 引用本文： | 邱峰,陈世知,陈虹.新型腺病毒载体的制备及其特性研究[J].重庆医科大学学报,2006,31(3):330-333. |
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| 作者姓名： | 邱峰 陈世知 陈虹 |
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| 作者单位： | 重庆医科大学附属第一医院临床药理研究室,重庆,400016;重庆医科大学附属第二医院核医学科,重庆,400010 |
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| 摘 要： | 目的：本研究在普通腺病毒载体（Adv）纤毛头节区插入了具有整合素特异性的RGD肽,制备了一种新型RGD-Adv,以克服Adv基因转导的柯萨奇腺病毒受体依赖性.方法：用体外连接法构建RGD-Adv,并考察其基因转效率、稳定性及体外、体内毒性.结果：RGD-Adv可有效地实现向受试细胞转导基因,其转导能力随病毒量的增加而加强.结论：具有av亲和性的RGD-Adv是一种安全高效的新型腺病毒载体,将在基因治疗及实验研究中发挥重要作用.
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| 关 键 词： | 腺病毒载体 RGD肽 整合素 |
| 文章编号： | 0253-3626(2006)03-0330-04 |
| 收稿时间： | 10 16 2005 12:00AM |
| 修稿时间： | 2005年10月16 |
Generation and characterization of fibre modified adenovirus vectors |
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| QIU Feng, et al.Generation and characterization of fibre modified adenovirus vectors[J].Journal of Chongqing Medical University,2006,31(3):330-333. |
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| Authors: | QIU Feng |
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| Institution: | Department of Clinical Pharmacology Lab, the First Affiliated Hospital, Chongqing Medical University |
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| Abstract: | Objective: In the present study,We developed and assess an integrin-targeted fiber-mutant Ad vector containing the Arg-Gly-Asp(RGD) peptide motif on the fiber knob for the transfection of melanoma cell lines.Methods: We constructed RGD-Adv by in vitro ligation method,and investigated the efficiency of gene transdution in melanoma cells,studied its stability,in vitro,and in vivo toxicology.Results: For in vivo and in vitro,gene transfer the RGD-Adv more efficient than the vector containing wild type fiber via an CAR independent cell entry pathway.Conclusion: These results revealed that gene delivery by RGD-Adv was more efficient than that by conventional Adv and suggest that av-integrin-targeted Adv will be of great utility for gene therapy and gene transfer experiments. |
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| Keywords: | Adenovirus vector RGD peptid Integrin |
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