应用基因表达谱技术研究人脑动脉瘤细胞信号传导相关基因

应用基因芯片分析人脑动脉瘤基因表达谱 ,分析细胞信号传导相关基因的差异表达。提取 4例人脑动脉瘤标本及作为正常对照的人颅底动脉环标本总RNA、mRNA ,进行线性扩增 ,通过逆转录的方法标记、制成cDNA探针 ,探针与84 6 4点基因表达谱芯片杂交 ,扫描芯片荧光信号图像 ,分析比较差异表达的基因。结果 :在 84 6 4条基因中 ,有 15条细胞信号传导相关基因表达有显著差异 ,其中表达上调 13条 ,表达下调 2条。提示 :差异表达的细胞信号传导相关基因及其参与的病理过程与脑动脉瘤的病理发生有关。

Differential Expression of Signal Transduction Related Genes on Human Cerebral Aneurysms

The aim was to study the expression of signal transduction related genes on huma n cerebral aneurysm s with microarray gene chip. 8 464 genes of the PCR products were spotted onto a glass plates in array. DNAs were fixed onto the glass plate. The total RNAs wer e isolated from the tissue, and were purified to mRNAs by Oligotex. Both mRNAs f rom the 4 human cerebral aneurysms and normal human Willis circle arteries were reversely transcribed to cDNAs with hybridization probes using the incorporation of Cy3-dCTP and Cy5-dCTP. The mixed probes were hybridized to the cDNA micr oarray. The cDNA microarray was scanned for the fluorescent signals and showed d ifferences between aneurysm and normal control. Among the 8 464 target genes, 1 5 signal transduction related genes showed significant different expression betw een aneurysms and normal control with 13 genes upregulated and 2 genes downregul ated. The differentially expressed genes and related pathology can play a key role in the pathogenesis of cerebral aneurysms.