黄芩不同炮制饮片的红外光谱特征分析

目的:以黄芩苷为对照,对黄芩3种饮片(生黄芩、酒黄芩、黄芩炭)的多级红外特征图谱进行比较分析,为建立黄芩不同饮片无损、快速的鉴别方法提供参考。方法:采用红外光谱与二维相关红外光谱技术,比较黄芩不同炮制饮片的红外图谱,发现其变化规律。结果:在一维红外光谱中,各饮片的谱图十分相似;通过分析二阶导数光谱发现,炒炭之后1 357cm-1吸收峰向高波数位移,1 296 cm-1吸收峰向低波数位移;而生黄芩、酒黄芩中的1 413(1 408)cm-1峰,在黄芩炭中消失。二维红外谱图显示特征峰差异明显,在800~1 300 cm-1波段,生黄芩有3个特征峰,1 078 cm-1为最大吸收峰;酒黄芩有4个特征峰,1 066 cm-1为最大吸收峰;黄芩炭有3个特征峰,1 129 cm-1为最大吸收峰;在1 300~1 800 cm-1波段,各饮片均呈现出4个特征峰,其中最大吸收峰生黄芩为1 576 cm-1,酒黄芩为1 620 cm-1,黄芩炭为1 558 cm-1。结论:多级红外特征图谱法可用于快速有效地分析和鉴定黄芩不同饮片,为深入诠释黄芩饮片的炮制原理提供参考。

Analysis of Different Processed Products of Scutellariae Radix Using Infrared Spectroscopy Technique

Objective: To identify three pieces of Scutellariae Radix (crude,processed by wine and fried into carbon) through infrared spectroscopy technique with baicalin as a standard. Method: 1D and 2D infrared spectroscopy were adopted to clarify potential change variation of three pieces of Scutellariae Radix by comparing their infrared spectra characteristic. Result: Spectra of three products were similar in 1D infrared spectroscopy.In the second derivative spectra,absorption peaks at 1357 cm^-1 and 1296 cm^-1 were moved to higher and lower wavenumbers after charred,respectively;absorption peak at 1413 (1408) cm^-1 in crude and processed by wine were disappeared after charred.There were significant differences in 2D infrared spectroscopy.During 800-1300 cm^-1,three characteristic peaks were shown in crude products with the strongest peak was at 1078 cm^-1;processed products by wine had four characteristic peaks with the strongest peak was at 1066 cm^-1;the strongest peak was at 1129 cm^-1 in fried into carbon products which had three characteristic peaks.Four characteristic peaks were shown between 1300-1800 cm^-1,the strongest peaks of crude,processed by wine and fried into carbon products were at 1576,1620,1558 cm^-1,respectively. Conclusion: Infrared spectroscopy technique can provide a great deal of information,which can provide a scientific basis for further explanation of change variation of processed products of Scutellariae Radix.